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Plasmid#154010PurposeExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
UseTagsHA tag, His6, and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG5-P190
Plasmid#31285DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-PytR(A2.1)-mCherry
Plasmid#204869PurposeExpresses pyrrolysyl tRNA variant "A2.1" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertM. mazei pyrrolysyl tRNA mutant "A2.1"
UseAAVTagsExpressionMammalianMutationU25C; A3G, A4G, T65C, T66C, T67TPromoterU6Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR3-Tango
Plasmid#66240PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCCR3 (CCR3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ICAM5-bio-His
Plasmid#51759PurposeExpresses full-length Intercellular adhesion molecule 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertICAM5 (ICAM5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NfiA
Plasmid#64901PurposeInducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.DepositorInsertNfia (Nfia Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3F-COMP5AP-AviTag-9xHis
Plasmid#157541PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3F (SEMA3F Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-Nono-sh5
Plasmid#127651PurposeKnock-down of human NONODepositorInsertNONO shRNA (NONO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaMKIV-DCTK75E
Plasmid#126423PurposeA kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertcalcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
UseTagsFLAGExpressionMammalianMutationchanged lysine 75 to glutamic acidPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1
Plasmid#114052Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1 (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance)PromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3C-COMP5AP-AviTag-9xHis
Plasmid#157525PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3C (SEMA3C Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSV-CaMKIV(1-313)
Plasmid#45063PurposeExpression of constit active CaMKIVDepositorInsertCam Kinase IV(1-313) (Camk4 Mouse)
UseTagsExpressionMammalianMutationtruncated at amino acid 313*PromoterRSVAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCcnb1
Plasmid#162793PurposeCcnb1 shRNA in pMKO.1 retroviral vectorDepositorInsertCcnb1 shRNA (Ccnb1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LAIR2-COMP5AP-AviTag-9xHis
Plasmid#157085PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertLAIR2 (LAIR2 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VSTM2L-COMP5AP-AviTag-9xHis
Plasmid#157146PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertVSTM2L (VSTM2L Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-p27Kip1 3'UTR
Plasmid#29476DepositorInsertp27 Kip1 3'UTR (CDKN1B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3G-COMP5AP-AviTag-9xHis
Plasmid#157539PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3G (SEMA3G Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep70
Plasmid#136821PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep70 (CEP70 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-dKARD
Plasmid#114053Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-dKARD (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance): AGATACTAGCT…PromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Egr3-EYFP-WPRE
Plasmid#186417PurposeOverexpression of Egr3 transcript variant 1 CDS under EF-1α promoterDepositorInsertEarly growth response 3 (Egr3 Mouse)
UseAAVTagsEYFPExpressionMutationPromoterEF-1-alphaAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only