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pSET-dCas9-actII-4-NT-S1

Plasmid

#110185

Purpose

Repression of gene expression in Streptomyces by CRISPRi

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Inserts

dCas9
sgRNA

Use

CRISPR; I

Tags

Expression

Bacterial

Mutation

D10A, H840A

Promoter

J23119 and ermE*p

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

Plasmid

#158963

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Article

Kesavan et al Development. 2020 Jun 8;147(11). pii: de

Inserts

hsp70l promoter
floxed Non-FP cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-IRAlus-Apobec3G

Plasmid

#92349

Purpose

Plasmid for expression of GFP gene with a Apobec3G gene intron derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Apobec3G gene intron derived IR Alu element containing 3'UTR (APOBEC3G Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Plasmid

#92390

Purpose

expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

Plasmid

#191369

Purpose

Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dPba3Cas13b-3xGFP-NLS

Use

Tags

3xGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Plasmid

#191537

Purpose

Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti‐puro‐OgRNA_mdxE53

Plasmid

#187054

Purpose

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alus-Apobec3G

Plasmid

#92348

Purpose

Plasmid for expression of GFP gene with a Apobec3G gene intron derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Apobec3G gene intron derived Alu element containing 3'UTR (APOBEC3G Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gαi*-BERKY3

Plasmid

#158424

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Use

Lentiviral

Tags

myc

Expression

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Expression

Mutation

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Ccas9LacZ

Plasmid

#113083

Purpose

Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCLucIPZ

Plasmid

#53222

Purpose

Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Cypridina luciferase
non-silencing shRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSF11-wElectra1

Plasmid

#184932

Purpose

Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1

Use

Tags

Expression

Worm

Mutation

Promoter

tag-168

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P09

Plasmid

#120020

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA180

Plasmid

#128538

Purpose

Expresses E coli Bir A protein

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

E coli BirA

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

Plasmid

#205261

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE163

Plasmid

#118511

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-HASAP1-ST

Plasmid

#138965

Purpose

chemigenetic voltage indicator, soma targeted

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HASAP1-ST

Use

Tags

somatic localization tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23111

Plasmid

#113149

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Insert

dCas9

Use

Tags

Expression

Bacterial

Mutation

D10A & H840A

Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUB-cHyPer2

Plasmid

#84728

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2

Use

Tags

Expression

Plant

Mutation

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCF CREB M1

Plasmid

#22969

Depositor

Marc Montminy

Article

Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

Insert

CREB (Creb1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Promoter

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRE165

Plasmid

#118513

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAdEasy Flag GCN5

Plasmid

#14106

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

E189G and K195N

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hEFA6B

Plasmid

#206413

Purpose

Mammalian expression of hEFA6B

Depositor

Hye-Won Shin, Kazuhisa Nakayama

Article

Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

Insert

EFA6B (PSD4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sg-mGreenLantern/EGFP

Plasmid

#179913

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/V5-His-TOPO-mir17-92

Plasmid

#21109

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

miR-17-92 cluster (MIR17 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV10-hTti1

Plasmid

#30213

Depositor

Noboru Mizushima

Article

Kaizuka et al J Biol Chem. 2010 Jun 25. 285(26):20109-

Insert

Tel2 interacting protein 1 (TTI1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z05

Plasmid

#120048

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DYSF-3HA

Plasmid

#29767

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF (DYSF Human)

Use

Tags

3xHA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
ptdEos-CLC

Plasmid

#38009

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Inserts

Clathrin, light polypeptide (LCa) (Clta Mouse)
Eos FP, tandem dimer

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

Plasmid

#192683

Purpose

Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP91

Plasmid

#83548

Purpose

Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PGK1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRE131

Plasmid

#118485

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TB:7xE-Box:MiniP

Plasmid

#133880

Purpose

7 copies of the E-box motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies of the E-box motif

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAIP Nup62Fv2GFP

Plasmid

#139684

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an SFFV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z34

Plasmid

#120062

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLIPE-100u

Plasmid

#13543

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
His-GFP-Granuphilin

Plasmid

#170867

Purpose

Mammalian expression of His-GFP-Granuphilin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Synaptotagmin-like 4 (SYTL4 Human)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-hTRAF3

Plasmid

#44032

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Use

Tags

1xHA

Expression

Mammalian

Mutation

S393G

Promoter

Available since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
pMRE140

Plasmid

#118492

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 WT MYOC eGLuc2 FLAG

Plasmid

#192820

Purpose

Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

Depositor

John Hulleman

Article

Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

Insert

myocilin (MYOC Human)

Use

Tags

FLAG and enhanced Gaussia luciferase (M43I/M110I)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP92

Plasmid

#83549

Purpose

Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TDH3 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
sc AAV miR26a eGFP

Plasmid

#21894

Depositor

Joshua Mendell

Article

Kota et al Cell. 2009 Jun 12. 137(6):1005-17.

Insert

EF1 alpha -miR26a GFP p(A)

Use

AAV; Sc

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 RagA shRNA #1

Plasmid

#30319

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagA shRNA (RRAGA Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
pMRE146

Plasmid

#118498

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBADmod1-linker2-gamS

Plasmid

#45833

Depositor

Richard Murray, Vincent Noireaux

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

gamS

Use

Synthetic Biology

Tags

Histag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCX-Klf4

Plasmid

#66656

Purpose

For expression of mouse Klf4.

Depositor

Barak Cohen

Article

Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

Insert

Klf4 (Klf4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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