Addgene

Showing: 1541 - 1560 of 145339 results

Results per page:

Synapsin sHRPa-NRX in FSW lentiviral vector

Plasmid

#73147

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Use

Lentiviral

Tags

2x HA tag and Flag tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Availability

Academic Institutions and Nonprofits only

Enlarge
Synapsin sHRPb-NLG in FSW lentiviral vector

Plasmid

#73148

Purpose

Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-NLG (Nlgn1 Synthetic, Mouse)

Use

Lentiviral

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay sHRPa-KDEL

Plasmid

#73149

Purpose

Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-KDEL

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay sHRPb-KDEL

Plasmid

#73150

Purpose

Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-KDEL

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
YIP sHRPa-Aga1P

Plasmid

#73151

Purpose

Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-Aga1P

Use

Tags

V5 tag

Expression

Yeast

Mutation

Promoter

glyceraldehyde-3-phosphate dehydrogenase GPD prom…

Availability

Academic Institutions and Nonprofits only

Enlarge
pCTCON2 Aga2P-HRP

Plasmid

#73152

Purpose

Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Aga2P-HRP

Use

Tags

HA tag and myc tag

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11_DHNTPase

Plasmid

#73154

Purpose

Expresses in E. coli, E. coli Dihydroneopterin triphosphate pyrophosphatase

Depositor

Maurice Bessman, Sandra Gabelli

Article

Gabelli et al Structure. 2007 Aug;15(8):1014-22.

Insert

ORF17_DHNTPase_NUD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pET11b_MutT

Plasmid

#73155

Purpose

Expresses in E. coli, E coli MutT

Depositor

Maurice Bessman, Sandra Gabelli

Article

Bessman et al J Biol Chem. 1996 Oct 11;271(41):25059-6

Insert

MutT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pET24a_UDP_Xase

Plasmid

#73158

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pET24a_UDP_Xase_E113

Plasmid

#73159

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113A

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Use

Tags

Expression

Bacterial

Mutation

change glutamate 113 to alanine

Promoter

Availability

Academic Institutions and Nonprofits only
pET24a_UDP_Xase_E162

Plasmid

#73160

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Use

Tags

Expression

Bacterial

Mutation

change glutamate 162 to alanine

Promoter

Availability

Academic Institutions and Nonprofits only
pET24a_CDP-CHase

Plasmid

#73161

Purpose

Expresses in E. coli, Bacillus cereus NP_831800 CDP-CHase

Depositor

Maurice Bessman, Sandra Gabelli

Article

Duong-Ly et al J Bacteriol. 2011 Jul;193(13):3175-85. d

Insert

pET24a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pSLC-217

Plasmid

#73163

Purpose

Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-relE (relE E. coli (bacteria))

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrhaB

Availability

Academic Institutions and Nonprofits only

Enlarge
Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Article

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Use

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDZ584 pKAN 1x-mCherry

Plasmid

#73172

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 1x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Use

Unspecified

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid

#73173

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Use

Tags

Expression

Mammalian and Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pZW1-snoVector

Plasmid

#73174

Purpose

Nuclear expression of RNAs

Depositor

Ling-Ling Chen

Article

Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

Inserts

SNORD116-13 (SNORD116-13 Human)
SNORD116-14 (SNORD116-14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Use

Tags

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b His6-FKBPF36V

Plasmid

#73180

Purpose

Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

Depositor

Thomas Wandless

Article

Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

Insert

FKBP(F36V) (FKBP1A Human)

Use

Tags

His 6x

Expression

Bacterial

Mutation

F36V

Promoter

Availability

Academic Institutions and Nonprofits only
pEF5B-FRT-cilia-APEX

Plasmid

#73186

Purpose

Expresses cilia-APEX in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

cilia-APEX (Nphp3 Glycine max, Mouse)

Use

Tags

internal GFP tag

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

Enlarge