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Plasmid#154281PurposeEntry vector encoding non-tagged O-GlcNAc TransferaseDepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationWT OGTPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
UseTagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 …PromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCB306-GFP-Rab8A
Plasmid#198470Purposelentiviral expression of GFP-Rab8ADepositorInsertGFP-Rab8A (RAB8A Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A
Plasmid#115171PurposeExpresses membrane-targeted Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmembrane targeting sequence and mycExpressionMammalianMutationFirst 32 amino acids of Sema4A replaced with memb…PromoterCMVAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-CD4-Sema4D
Plasmid#51603Purposeexpresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4DepositorInsertCD4-Sema4D
UseTagsmycExpressionMammalianMutationSema4D amino acids 1-754 have been replaced with …PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKP4010
Plasmid#113336PurposeBeta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertsArtificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase
UseTags3X-FLAG/8X-Gly linkerExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRIG-myc-Sema4DdeltaC
Plasmid#51607Purposeexpresses myc-tagged Sema4D lacking C terminus in lentiviral backboneDepositorInsertSema4DdeltaC (Sema4d Mouse)
UseLentiviralTagsmyc tagExpressionMammalianMutationdeleted last 70 amino acids in the C-terminusPromoterRSVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CSII-PPM1H-mApple-mito
Plasmid#207351PurposeMammalian expression of PPm1H-mitoDepositorInsertPPm1H-mito (PPM1H Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-PPM1H S124A
Plasmid#208368PurposeMammalian expression of HA-tagged human PPM1H S124ADepositorInsertHA-PPM1H S124A (PPM1H Human)
UseTagsExpressionMammalianMutationS124APromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-PPM1H S211A
Plasmid#208369PurposeMammalian expression of HA-tagged human PPM1H S211ADepositorInsertHA-PPM1H S211A (PPM1H Human)
UseTagsExpressionMammalianMutationS211APromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-Rab12 Q101L
Plasmid#207347PurposeBacterial expression of Rab12DepositorInsertRab12 (RAB12 Human)
UseTags2xHisExpressionBacterialMutationQ101LPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO PPM1H
Plasmid#207333PurposeBacterial expression of His SUMO PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-L2D/V9D/I19D PPM1H
Plasmid#207341PurposeMammalian expression of HA-L2D/V9D/I19D PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHAExpressionMammalianMutationL2D/V9D/I19DPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Rab10(Q68L)
Plasmid#207342PurposeBacterial expression of His SUMO Rab10(Q68L)DepositorInsertRab10 (RAB10 Human)
UseTagsHis-SUMOExpressionBacterialMutationQ68LPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mYwhae -2
Plasmid#198498Purposelentiviral stable expression of mYwhae gRNA 2DepositorInsertmYwhae (Ywhae Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mYwhae - 1
Plasmid#198497Purposelentiviral stable expression of mYwhae gRNA 1DepositorInsertmYwhae (Ywhae Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S69A/S241A/S308A/S334A
Plasmid#51594Purposeexpresses RNAiR myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerines 69, 241, 308, 334 to Alanines; also RNAi …PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSICO-eGFP-TNPO3
Plasmid#167590PurposeExpresses human TNPO3 fused to eGFPDepositorInserteGFP-TNPO3 (TNPO3 Human)
UseLentiviralTagseGFPExpressionMutationWTPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A FKBP12F36V-OGT
Plasmid#154287PurposeEntry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x HA and FKBP12(F36V)ExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only