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Plasmid#129282PurposeExpression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)DepositorInsertalpha-Xylp_1211 (Ga0256695_ 1211)
UseTagsExpressionBacterialMutationPromoterClostridium thermocellum DSM1313 Cellobiose Phosp…Available sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZSCAN18-donor
Plasmid#113821PurposeCRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFPDepositorInsertZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-hTie1-ECD-FLAG
Plasmid#119235PurposePlasmid encodes FLAG-tagged extracellular domain of human Tie1 gene cloned in lentiviral vector.DepositorInsertTIE1-ECD-FLAG (TIE1 Human)
UseLentiviralTagsFLAGExpressionMutationContains extracellular domain (ECD) onlyPromoterP hUbCAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF561-donor
Plasmid#113801PurposeCRISPR donor plasmid to tag human transcription factor ZNF561 with GFPDepositorInsertZNF561 homology arms flanking EGFP-IRES-Neo cassette (ZNF561 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF700-donor
Plasmid#112385PurposeCRISPR donor plasmid to tag human transcription factor ZNF700 with GFPDepositorInsertZNF700 homology arms flanking EGFP-IRES-Neo cassette (ZNF700 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF169-donor
Plasmid#112376PurposeCRISPR donor plasmid to tag human transcription factor ZNF169 with GFPDepositorInsertZNF169 homology arms flanking EGFP-IRES-Neo cassette (ZNF169 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZFP1-donor
Plasmid#112374PurposeCRISPR donor plasmid to tag human transcription factor ZFP1 with GFPDepositorInsertZFP1 homology arms flanking EGFP-IRES-Neo cassette (ZFP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF227-donor
Plasmid#112368PurposeCRISPR donor plasmid to tag human transcription factor ZNF227 with GFPDepositorInsertZNF227 homology arms flanking EGFP-IRES-Neo cassette (ZNF227 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTX1-donor
Plasmid#112364PurposeCRISPR donor plasmid to tag human transcription factor OTX1 with GFPDepositorInsertOTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF175-donor
Plasmid#112347PurposeCRISPR donor plasmid to tag human transcription factor ZNF175 with GFPDepositorInsertZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF354C-donor
Plasmid#112339PurposeCRISPR donor plasmid to tag human transcription factor ZNF354C with GFPDepositorInsertZNF354C homology arms flanking EGFP-IRES-Neo cassette (ZNF354C Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF347-donor
Plasmid#112326PurposeCRISPR donor plasmid to tag human transcription factor ZNF347 with GFPDepositorInsertZNF347 homology arms flanking EGFP-IRES-Neo cassette (ZNF347 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pRP-iSL(MCS1+2)
Plasmid#69245PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFREE
Plasmid#92050PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionBacterialMutationPromoterpRhamBAD and ptetAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA (V5 and HA)
Plasmid#222485PurposeCo-expression of two genes in yeast with V5 and HA tags and URA3 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TRP (V5 and HA)
Plasmid#222488PurposeCo-expression of two genes in yeast with V5 and HA tags and TRP1 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-HIS (V5 and HA)
Plasmid#222487PurposeCo-expression of two genes in yeast with V5 and HA tags and HIS3 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU (V5 and HA)
Plasmid#222486PurposeCo-expression of two genes in yeast with V5 and HA tags and LEU2 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only