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Plasmid#164218PurposeExpresses mEmerald tagged CAPRIN1 under EF-1a promoterDepositorInsertCAPRIN1 (CAPRIN1 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF-1aAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBFC1207
Plasmid#186694PurposeVcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right EndDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
UseTagsExpressionBacterialMutationPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLEX-PGK-LAMP1-HaloTag
Plasmid#164216PurposepLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsHaloTagExpressionMutationPromoterPGKAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBFC0996
Plasmid#186695PurposeVcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right EndDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
crRNA
Tn7 transposon
2xLguI Cargo Stuffer
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
UseTagsExpressionBacterialMutationPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGJK_His-SUMO-LbuCas13a
Plasmid#172488PurposeBacterial expression of codon-optimized His-SUMO-LbuCas13a.DepositorInsertbdSUMO-LbuCas13a (codon-optimized)
UseTagsHis6 and bdSUMOExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLEX-PGK-ANXA11-mCerulean
Plasmid#164214PurposepLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmCeruleanExpressionMutationPromoterPGKAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_His-TEV-EiCsm6
Plasmid#172487PurposeBacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.DepositorInsertEnterococcus italicus Csm6 (codon optimized)
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-smGFP-Kv2.1
Plasmid#131709PurposeExpresses Kv2.1 in mammalian cellsDepositorInsertKCNB1 (KCNB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11
Plasmid#164203PurposeExpresses CRY2oligo-mcherry tagged ANXA11 under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(R235Q)
Plasmid#164205PurposeExpresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutationR235QPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(D40G)
Plasmid#164204PurposeExpresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutationD40GPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-SUMO-TEV-TtCsm6
Plasmid#172486PurposeBacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.DepositorInsertT. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
UseTagsHis-SUMO-TEVExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(R346C)
Plasmid#164206PurposeExpresses CRY2oligo-mcherry tagged ANXA11 R346C mutant under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutationR346CPromoterCMVAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)
Plasmid#164208PurposeExpresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutation186-505 aaPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(1-185aa)
Plasmid#164207PurposeExpresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutation1-185 aaPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald R346C
Plasmid#164213PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationR346CPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald R235Q
Plasmid#164212PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationR235QPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald D40G
Plasmid#164211PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationD40GPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only