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pv7_MBD1_MBD_noEGFP

Plasmid

#179406

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pv7_CBX1_2xChromo_noEGFP

Plasmid

#179395

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo_noEGFP

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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R6K_tac_dGFPmut3_ChInt

Plasmid

#187388

Purpose

chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)

Depositor

Roi Avraham

Insert

dGFPmut3_AAV

Use

Tags

AAV

Expression

Bacterial

Mutation

C-terminal AAV tail

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph18_eGFP_CCR5TC

Plasmid

#187443

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph15_eGFP_CCR5TC

Plasmid

#187442

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP1D5

Plasmid

#165063

Purpose

Characterisation of yeast promoter using yEGFP as reporter gene and PGK1 terminator

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_K490_GFP_SNAP

Plasmid

#159721

Purpose

Expresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (only amino acids 1-490), K490 (KIF5B Human)

Use

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Mutation

Only amino acids (1-490)

Promoter

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_EGFP_Nick_Dual_sgRNA

Plasmid

#178095

Purpose

Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + EGFP nick sgRNAs

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_eGFP

Plasmid

#136095

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12_eGFP

Plasmid

#136085

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_eGFP(noStop)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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DDX41_p.L237F_p.P238T_EGFP

Plasmid

#175845

Purpose

Expresses DDX41 with mutation

Depositor

Evan Braunstein

Article

Li et al Haematologica. 2016 Jun;101(6):e228-31.

Insert

DDX41 (DDX41 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

c.711G>T, p.L237F and c.712C>A, p.P238T

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169318

Purpose

Doxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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psfGFP

Plasmid

#165829

Purpose

sfGFP green fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

sfGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.EFS.eGFP.pre

Plasmid

#169276

Purpose

Constitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJH120_pE12_HyPep_emGFP

Plasmid

#160406

Purpose

Gateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySyn

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyPep emGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

R07E5.16 (U6)

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF143_SECGFP_sg1

Plasmid

#163864

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

R07E5.16 (U6)

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP1H5

Plasmid

#165065

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFPY2A

Plasmid

#165066

Purpose

Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP105

Plasmid

#165060

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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