-
Plasmid#205636PurposereRNA guide targeting Mus Musculus SCN9ADepositorInsertreRNA-mmSCN9A (Scn9a Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
hCD12
Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET mNG-GECO1
Plasmid#201933PurposeExpresses mNG-GECO1 indicator in bacteriaDepositorInsertmNeonGreenGECO1
UseTagsHIS tagExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN378 - pTRV2-CrGLK
Plasmid#203888PurposeViral silencing plasmid targeting the CrGLK gene from C. roseus.DepositorInsertCrGLK fragment
UseRNAiTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
H1.99.M.luc
Plasmid#193664PurposeExpresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99) with the TATA box mutatedDepositorInsertTruncated Pol III H1 promoter with a mutated TATA box (H1.99m) + N41
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV1:T35s
Plasmid#164738PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr6g043230.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV9:T35s
Plasmid#164742PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr4g116360.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV10:T35s
Plasmid#164739PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr8g446520.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP
Plasmid#168491PurposeMammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertthe E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19_4xT790_28bp
Plasmid#114720Purpose4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T790_28bp
Plasmid#114722Purpose28 bp fragment of the WT EGFR allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_Strep
Plasmid#101651PurposeExpression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_Strep
Plasmid#101652PurposeExpression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-ATF7IPi1
Plasmid#59614PurposeExpression of shRNA against human ATF7IPDepositorInsertATF7IP (ATF7IP Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-ATF7IPi2
Plasmid#59615PurposeExpression of shRNA against human ATF7IPDepositorInsertATF7IP (ATF7IP Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
UseTagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertGEM-GECO
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam APC
Plasmid#16507DepositorInsertAPC (APC Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Caveolin-C-10
Plasmid#55468PurposeLocalization: Membrane, Excitation: 462, Emission: 492DepositorInsertNM_001753 (CAV1 Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 pcDNA3
Plasmid#206072Purposeexpression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expressionDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only