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Plasmid#159721PurposeExpresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (only amino acids 1-490), K490 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationOnly amino acids (1-490)PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EGFP_Nick_Dual_sgRNA
Plasmid#178095PurposeControl vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EGFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP
Plasmid#136095PurposeFP for C-term fusion with CDS12DepositorInsertCTAG_eGFP(noATG)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDS12_eGFP
Plasmid#136085PurposeFP for N-term fusion with a CTAGDepositorInsertCDS12_eGFP(noStop)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
DDX41_p.L237F_p.P238T_EGFP
Plasmid#175845PurposeExpresses DDX41 with mutationDepositorInsertDDX41 (DDX41 Human)
UseTagsEGFPExpressionMammalianMutationc.711G>T, p.L237F and c.712C>A, p.P238TPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#169318PurposeDoxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent proteinDepositorInsertArid3a (Arid3a Mouse)
UseLentiviralTagsIRES-dTomatoExpressionMutationPromoterTREAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
psfGFP
Plasmid#165829PurposesfGFP green fluorescent protein CDSDepositorInsertsfGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.pre
Plasmid#169276PurposeConstitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH120_pE12_HyPep_emGFP
Plasmid#160406PurposeGateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySynDepositorInsertpDONR221 HyPep emGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF144_SECGFP_sg2
Plasmid#163865PurposesgRNA targeting GFP in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting GFP
UseCRISPRTagsExpressionWormMutationPromoterR07E5.16 (U6)Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF143_SECGFP_sg1
Plasmid#163864PurposesgRNA targeting GFP in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting GFP
UseCRISPRTagsExpressionWormMutationPromoterR07E5.16 (U6)Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1H5
Plasmid#165065PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPY2A
Plasmid#165066PurposeCharacterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFPDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP105
Plasmid#165060PurposeIntegrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP104
Plasmid#165059PurposeIntegrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54_mEGFP494(-6)
Plasmid#163434PurposeMammalian expression of Nup54 with a mEGFP (missing the first 6 amino acids) at amino acid position 494DepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-9b)
Plasmid#163420PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-8b)
Plasmid#163418PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBz172_CsoS2_N1_GFP
Plasmid#140852PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backboneDepositorInsertCsoS2 NTD repeat 1 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz33_CsoS2_NTD_GFP
Plasmid#140861PurposeHis-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFPDepositorInsertCsoS2 NTD fused to GFP
UseTagsHexahistidine tag and Superfolder GFPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only