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Showing: 3041 - 3080 of 7564 results

  12. pOCC175

    Plasmid
    #118890
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease; mGFP, cleav…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118890-plasmid-map-sequence-id-232288

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  13. HsSPHK1

    Plasmid
    #118091
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    118091-plasmid-map-sequence-id-234124

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  15. pOCC267

    Plasmid
    #118887
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease; HALO tag
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118887-plasmid-map-sequence-id-232249

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  18. pCDF-NtXNtT2R1AtR2NtB2

    Plasmid
    #160886
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T2: T7, chaperones: T9
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160886-plasmid-map-sequence-id-318849

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  20. MmSphk1

    Plasmid
    #118599
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Mus musculus sphingosine kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    118599-plasmid-map-sequence-id-234126

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  39. pSC201

    Plasmid
    #129387
    Purpose
    A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.
    Depositor
    Silvia Cardona
    Article
    Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do
    Insert
    oriR6K and oriT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    N/A
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    129387-plasmid-map-sequence-id-257366

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  40. pOCC120

    Plasmid
    #118892
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and mGFP, HIS6, c…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118892-plasmid-map-sequence-id-232375

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Showing: 3041 - 3080 of 7564 results