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Plasmid#149282PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_mFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E347 Hs.TIAM1
Plasmid#70631PurposeGateway ORF clone of human TIAM1 [NM_003253.2] with stop codon (for native or N-terminal fusions)DepositorInsertTIAM1 (TIAM1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-3xFLAG-WPRE
Plasmid#127862PurposepAAV plasmid expressing 3xFLAG under the hSyn promoterDepositorInsert3xFLAG
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-BRSK2
Plasmid#23432DepositorInsertBRSK2 (BRSK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
LB1353 (Bcl-2 promoter, no IgH enh)
Plasmid#15393DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 with full promoter and no IgH enhancers MHS…PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
GWF297
Plasmid#133612PurposeTET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFPDepositorInsertTET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIT5_KT
Plasmid#173773PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT5_KH
Plasmid#173772PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP
Plasmid#75452PurposePlasmid for constitutive expression of gfpDepositorInsertgfp
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
AlphaC
Plasmid#124525PurposepColE1_AlphaCDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-TMPRSS2-TwinStrep_Zeo
Plasmid#192000PurposeLentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell lineDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsTMPRSS2ExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
R777-E222 Hs.RALGDS-nostop
Plasmid#70506PurposeGateway ORF clone of human RALGDS [NM_006266.3] without stop codon (for C-terminal fusions)DepositorInsertRALGDS (RALGDS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Omega Destination-CMV::ELuc:bGH
Plasmid#124528PurposeOmega destination vector, that has a CMV:ELUC:bgH in the backboneDepositorTypeEmpty backboneUseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_mKate2_synCoTC
Plasmid#199470PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
R777-E051 Hs.ECT2
Plasmid#70335PurposeGateway ORF clone of human ECT2 [NM_018098.5] with stop codon (for native or N-terminal fusions)DepositorInsertECT2 (ECT2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-Cdt1-mCherry
Plasmid#193760PurposeDoxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag)DepositorInsertCdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationWTPromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-ND-Cdt1-mCherry
Plasmid#193761PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradationDepositorInsertND-Cdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5xNF-kb_RE
Plasmid#118050PurposeGBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VP40
Plasmid#74421PurposeMammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusionDepositorInsertVP40
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLuciferase (GB0096)
Plasmid#68201PurposeProvides the CDS of the firefly luciferase protein as a level 0 GoldenBraid partDepositorInsertLuciferase coding region
UseLuciferase and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
R777-E229 Hs.RASA2
Plasmid#70513PurposeGateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)DepositorInsertRASA2 (RASA2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_SKIL
Plasmid#45289DepositorInsertSNON (SKIL Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL-Let7-perf
Plasmid#115367PurposeExpression vector to produce humanized Renilla luciferase with a single perfectly complementary binding site for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-ECFP
Plasmid#48353PurposeContributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertECFP
UseGateway entry vectorTagsExpressionMutationContains a stop codon.PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFIV-H1-Puro-hPolß
Plasmid#18663DepositorInsertshRNA specific to human DNA polymerase beta (POLB Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationnonePromoterAvailable sinceOct. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
AlphaB
Plasmid#124524PurposepColE1_AlphaBDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
AlphaD
Plasmid#124526PurposepColE1_AlphaDDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1-GFP
Plasmid#51407PurposeIs a biosensor for PI(4,5)P2DepositorInsertPH-PLCD1 (aa 1–170) (PLCD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcs2-GFP-IRES-mCherry
Plasmid#226098PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rac1(WT)
Plasmid#224278PurposeCell transfection and expression of Rac1DepositorInsertRac1 (RAC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-WT-T373A-S608A-S612A-puro
Plasmid#212674PurposeExpress tagged hRb mutantDepositorInsertRb (RB1 Human)
UseLentiviralTagsHAExpressionMutationT373A/S608A/S612APromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi
Plasmid#199808Purposevector for constitutive CRISPRi-mediated gene knockdown in S. aureusDepositorInsertsdCas9
sgRNA with GFP cassette
UseCRISPRTagsExpressionMutationPromoterP23 and PRAB17 (sgRNA) and SarAP1 (GFP)Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTripz Casp1-IRES-mCherry
Plasmid#187294PurposeThis is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).DepositorInsertCaspase1 (Casp1 Mouse)
UseLentiviralTagsmCherryExpressionMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHluorin
Plasmid#185537PurposeExpresses LGI1-pHluorin under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHluorinExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-ConVERGD-eGFP-WPRE
Plasmid#218745PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-CoffFon-eGFP-W3SL
Plasmid#218750PurposeProvides NOT intersectional (Flp NOT Cre) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMal-Strep
Plasmid#209345PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA43-GG
Plasmid#208014Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS8 AA 1-308
Plasmid#196905PurposeExpresses His-tagged Drosophila IntS8 amino acids 1-308DepositorInsertIntegrator subunit 8 (IntS8 Fly)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-ultraID-Zeo
Plasmid#207737PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits