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pMSCV-FLAG-2xSTREP-CCND1-Puro

Plasmid

#172643

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUCIDT-attL1-Worm ABeta-attR5

Plasmid

#160437

Purpose

Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human, Nematode)

Use

Tags

Cleavage signal

Expression

Bacterial

Mutation

Codon-optimized for expression in C. elegans

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-FFSS-Cyclin D1

Plasmid

#215148

Purpose

Expresses FFSS-Cyclin D1 in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

FFSS (FLAG-FLAG-STREP-STREP)

Expression

Mammalian

Mutation

Promoter

CMV (Tet inducible)

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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p21-mCerulean-PuroR

Plasmid

#215077

Purpose

Expresses p21-mCerulean in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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TurboID-13x-Cyclin D1

Plasmid

#215083

Purpose

Expresses TurboID-13x-Cyclin D1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

TurboID-13x

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-FFSS-Cyclin D1

Plasmid

#215146

Purpose

Expresses FFSS-Cyclin D1 in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

FFSS (FLAG-FLAG-STREP-STREP)

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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NLS-mAzGreen-Cyclin D1

Plasmid

#215071

Purpose

Expresses NLS-mAzGreen-Cyclin D1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

NLS-mAzGreen

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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NES-mAzGreen-Cyclin D1 (HP)

Plasmid

#215070

Purpose

Expresses NES-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

NES-mAzGreen

Expression

Mammalian

Mutation

Cyclin D1(HP) denotes mutations M56A, V60A, and W…

Promoter

LTR

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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NES-mAzGreen-Cyclin D1

Plasmid

#215069

Purpose

Expresses NES-mAzGreen-Cyclin D1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

NES-mAzGreen

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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NLS-mAzGreen-Cyclin D1 (HP)

Plasmid

#215072

Purpose

Expresses NLS-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

NLS-mAzGreen

Expression

Mammalian

Mutation

Cyclin D1(HP) denotes mutations M56A, V60A, and W…

Promoter

LTR

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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