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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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Piggybac-TetO-hNurr1-blast

Plasmid

#176485

Purpose

TetO-hNurr1 in a PiggyBac vector with Blasticidin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

Nurr1 (NR4A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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LeGO-S2

Plasmid

#85211

Purpose

Lentiviral vector expressing T-Sapphire fluorescent protein driven by SFFV promoter

Depositor

Boris Fehse

Article

Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

Insert

T-Sapphire

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-pAG-TM

Plasmid

#207318

Purpose

Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

protein AG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-SEP-GluR1

Plasmid

#24000

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

GluR1 (Gria1 Rat)

Use

Tags

GluR1 signal peptide (aa1-22) and SEP (supereclip…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pCXLE-hOCT3/4

Plasmid

#27076

Purpose

Non-integrating (episomal) expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

OCT3/4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Expression

Mutation

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
plenti hSyn PKM1

Plasmid

#220920

Purpose

Neuronal specific expression of PKM1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PKM1 (PKM Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCD86-EGFP

Plasmid

#133858

Purpose

Mammalian expression of CD86 C-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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1236 pcDNA3 Myr HA Akt3

Plasmid

#9017

Depositor

William Sellers

Insert

Akt3 (AKT3 Human)

Use

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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