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CD55-CD4d3+4-bio

Plasmid

#73116

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD55 (CD55 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-STChRger2-TS-EYFP

Plasmid

#129395

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Nup98-pDONR221

Plasmid

#124241

Purpose

Gateway entry clone of Drosophila Nup98-96 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Nup98-96 (Nup98-96 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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10XUAS IVS Jaws mVenus_tr

Plasmid

#111553

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mVenus_tr

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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AchE-CD4d3+4-bio

Plasmid

#73119

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding AchE with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

AchE (ACHE Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

Plasmid

#184738

Purpose

encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2

Depositor

Randall Platt

Article

Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

Insert

FsRT-Cas1(opt)-Cas2(opt)

Use

Tags

Expression

Bacterial

Mutation

sequence codon optimized for expression in E. coli

Promoter

pTetA

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS Jaws mCherry_tr

Plasmid

#111549

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Fyn deltaSH3

Plasmid

#16034

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Delta SH3: deletion of amino acids 76-141

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
ERMAP-CD4d3+4-bio

Plasmid

#73107

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ERMAP with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ERMAP (ERMAP Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_CEACAM1

Plasmid

#140102

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

gRNA1 under U6 and gRNA2 under H1

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pECE-M2-AMPKalpha2 M93G

Plasmid

#31653

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
AMIGO2-CD4d3+4-bio

Plasmid

#73103

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding AMIGO2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

AMIGO2 (AMIGO2 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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Lutheran-CD4d3+4-bio

Plasmid

#73101

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Lutheran with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Lutheran (BCAM Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Cln6

Plasmid

#78109

Purpose

mammalian expression of mouse Cln6 with C terminal EGFP fusion

Depositor

Thomas Braulke

Article

Thelen et al PLoS One. 2012;7(4):e35493. doi: 10.1371

Insert

Cln6 (Cln6 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
DSCAML1-CD4d3+4-bio

Plasmid

#73105

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding DSCAML1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

DSCAML1 (DSCAML1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDECKO_mCherry_Rat_cebp-alfa

Plasmid

#140098

Purpose

CRISPR-Cas9 library validation (positive control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

gRNA1 under U6 and gRNA2 under H1

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDECKO_mCherry_SPI1

Plasmid

#140099

Purpose

CRISPR-Cas9 library validation (positive control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

gRNA1 under U6 and gRNA2 under H1

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/TO-YOD1-Strep-HA

Plasmid

#113483

Purpose

Expression of human YOD1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

YOD1 (YOD1 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKalpha2 M93G

Plasmid

#31655

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

Plasmid

#184739

Purpose

encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106

Depositor

Randall Platt

Article

Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

Insert

FsRT-Cas1(opt)-Cas2(opt)

Use

Tags

Expression

Bacterial

Mutation

sequence codon optimized for expression in E. coli

Promoter

pTetA

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge