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Plasmid#187390Purposearabinose-inducible expression of GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D41
Plasmid#185841PurposeTesting the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EC4
Plasmid#185880PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EA4
Plasmid#185879PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EG3
Plasmid#185878PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4BG6
Plasmid#185877PurposeA parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AG4ARSd
Plasmid#185876PurposeA plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AG4
Plasmid#185874PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AE4
Plasmid#185873PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A5C
Plasmid#185872PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1DFB
Plasmid#185871PurposeA parental plasmid for in vivo gene amplification at RPL25 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B9)
Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1B
Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1A
Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationDHFR P66LPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_3EGFP
Plasmid#185967PurposeExpresses EGFP from PR promoter in E.coli Dh5alpha cellDepositorInsertPr-Egfp cassette in forward direction in Network Brick
UseSynthetic BiologyTagsExpressionMutationPromoterPRAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only