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Plasmid#196295Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSsDepositorInsertFull length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
UseCRISPRTagsSpeI-DR-BsaI-DR-SpeIExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNA0304
Plasmid#165612PurposeVector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)DepositorInsertSNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
UseCRISPRTagsExpressionYeastMutationNotI site replaced the CAN1 spacer in parent vect…PromoterSNR52Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX_EFS-Cre_ppt-del
Plasmid#162073PurposeCompact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEFS (part of insert)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECO200
Plasmid#169765PurposeExpress SpCas9 and Basta resistance geneDepositorTypeEmpty backboneUsePlant binary vectorTagsExpressionPlantMutationPromoter35S, AtU6Available sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECO300
Plasmid#169766PurposeExpress SpCas9 and Kanamycin resistance geneDepositorTypeEmpty backboneUsePlant binary vectorTagsExpressionPlantMutationPromoter35S, AtU6Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-empty-GFP
Plasmid#221843Purposeempty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertEGFP
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterCAGCAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCBLggPro
Plasmid#199736PurposeGolden Gate entry vector for promoter cloningDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLggCDS
Plasmid#199737PurposeGolden Gate entry vector for gene of interest cloningDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLggTerm
Plasmid#199738PurposeGolden Gate entry vector for terminator cloningDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWD509
Plasmid#73739PurposeGateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5DepositorInsert2xNLS-FLP-D5
UseTagsExpressionWormMutationG5DPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-Cas9-2A-puro
Plasmid#216390Purposethis vector can be used to make modRNA expressing Cas9 and puro resistance geneDepositorInsertCas9-2A-puro
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBUE411-GG
Plasmid#198232PurposeWheat GRF4-GIF1 chimera in pBUE411DepositorInsertGRF4-GIF1 chimera cassette
UseCRISPR; Plant binary vectorTagsExpressionPlantMutationPromoterUbiquitinAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBUE411-B
Plasmid#198234PurposepPLTP::BBM cassette in pBUE411DepositorInsertpPLTP::BBM cassette
UseCRISPR; Plant binary vectorTagsExpressionPlantMutationPromoterPLTPAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAC200
Plasmid#187911Purposepsac200-crispey-bar-vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194727PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.DepositorArticleInsertAsCpf1(D908A) (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGMV-gSWEET4
Plasmid#112796PurposeUniversal BeYDV-derived vector with gRNAs targeting the VviSWEET4 geneDepositorInsertgRNA pair targeting VviSWEET4
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194723Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1DepositorArticleInsertAsCpf1 (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReplace_donor_mcherry
Plasmid#149345PurposeDonor vector for the replacement of reporter BFP by mcherryDepositorInsertmcherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTC393
Plasmid#91221Purposeprotoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only