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pREP41xN_ccdB
Plasmid#79023PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP42xN_ccdB
Plasmid#79024PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
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mU6-sgAmpk1-hU6-sgAmpk2
Plasmid#177224PurposeExpresses Ampk1 and Ampk2 targeting gRNAs and Cre-recombinaseDepositorInsertsgPrkaa1/sgPrkaa2
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgSik1_2nd-hU6-sgSik3_2nd
Plasmid#177219PurposeExpresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgSik1_2nd/sgSik3_2nd
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgSik1_1st-hU6-sgSik3_2nd
Plasmid#177217PurposeExpresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgSik1_1st/sgSik3_2nd
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgSik3_2nd-hU6-sgNT
Plasmid#177215PurposeExpresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgSik3_2nd/sgNT1
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRFX7.1.0-gDNA
Plasmid#132455PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertRFX7 (RFX7 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-1883
Plasmid#188669PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX458_SSRP1_1
Plasmid#72371PurposeEncodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFPDepositorInsertSSRP1 (SSRP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCfb13220
Plasmid#219895PurposeThe base plasmid of TUNEYALI for TF30DepositorInsertContains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13194
Plasmid#219869PurposeThe base plasmid of TUNEYALI for TF04DepositorInsertContains gRNA targeting TF04 (YALI1_C02831g) and homologous arm matching TF04
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13196
Plasmid#219871PurposeThe base plasmid of TUNEYALI forTF06DepositorInsertContains gRNA targeting TF06 (YALI1_D34785g) and homologous arm matching TF06
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13231
Plasmid#219905PurposeThe base plasmid of TUNEYALI for TF41DepositorInsertContains gRNA targeting TF41 (YALI1_D00747g) and homologous arm matching TF40
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13213
Plasmid#219888PurposeThe base plasmid of TUNEYALI for TF23DepositorInsertContains gRNA targeting TF23 (YALI1_E32722g) and homologous arm matching TF23
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only