Showing: 1501 - 1520 of 1875 results
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Plasmid#186622PurposeEncodes Stim1 mutant D76A fused to YFPDepositorInsertStromal Interaction Molecule 1 (STIM1 Human)
UseTagsYFPExpressionMammalianMutationD76APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)
Plasmid#195741PurposeBase editing for making a pre-stop codon in mmoX using phenol inducible systemDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPo (phoenol-inducible promoter)AvailabilityAcademic Institutions and Nonprofits only -
Td-CBEmax
Plasmid#196600PurposeMammalian expression of Td-CBEmaxDepositorInsertbpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
UseTags6*HisExpressionMammalianMutationTadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ269E2
Plasmid#164721PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
UseCRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…TagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265F2
Plasmid#164722PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)NG-UGI
UseCRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…TagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265G2
Plasmid#164723PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-ziSpyMac(D10A)-UGI)
UseCRISPR; Gateway compatible a3a/y130f-ispymaccas9-…TagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEa-V106W
Plasmid#193838PurposeExpress TadCBEa-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa V106W-SpCas9 D10A
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd-V106W
Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pBE-t
Plasmid#195342PurposeBase editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110VDepositorInsertsecTadA(8e)-SpCas9-NG
gRNA ctctacgcgggtcttgtagt
UseCRISPRTagsExpressionMutationSpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…PromoterLac and ProCAvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pBE-nt
Plasmid#195343PurposeBase editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNADepositorInsertsecTadA(8e)-SpCas9-NG
gRNA gtgcacgacgccgtatgcga
UseCRISPRTagsExpressionMutationSpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…PromoterLac and ProCAvailabilityAcademic Institutions and Nonprofits only -
pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR
Plasmid#200639PurposeLow STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AXBEv2
Plasmid#204606PurposeMammalian expression of evolved adenine transversion base editor AXBEv2DepositorInsertbpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)PromoterAvailabilityAcademic Institutions and Nonprofits only -
ACBE
Plasmid#204607PurposeMammalian expression of adenine-to-cytosine base editor ACBEDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGFP-mTet1s_K852R pc3914
Plasmid#201704PurposeExpresses of GFP-tagged mTet1s_K852R in mammalian cellsDepositorInsertTet1s-K852R (Tet1 Mouse)
UseTagsGFPExpressionMammalianMutationLysine 852 mutated to argininePromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pGFP-mTet1s_K852E pc3915
Plasmid#201705PurposeExpresses of GFP-tagged mTet1s_K852E in mammalian cellsDepositorInsertTet1s-K852E (Tet1 Mouse)
UseTagsGFPExpressionMammalianMutationLysine 852 mutated to glutamatePromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pmch-mTet1-ZF-CD pc3956
Plasmid#201709PurposeExpresses of mcherry-tagged mTet1-ZF-CD in mammalian cellsDepositorInsertTet1-ZF_CD (Tet1 Mouse)
UseTagsmCherryExpressionMammalianMutationZinc finger domain of Tet1 fused to its catalytic…PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
SpRY dCas9 KRAB
Plasmid#188501PurposeExpresses FLAG tagged SpRY dCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
SpRY dCas9 VPR
Plasmid#188511PurposeExpresses FLAG tagged SpRY dCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pB80-LAMP1-mCherry-iLID
Plasmid#174625PurposeOptogenetic coupling to lysosomes (LAMP1) via iLIDDepositorInsertLAMP1-mCherry-iLID (LAMP1 Synthetic, Human)
UseTagsmCherry-iLIDExpressionMammalianMutationmCherry: Met1DelPromoterChicken beta-actinAvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5A-Rigor
Plasmid#172202PurposeExpresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5A (Kif5a Mouse)
UseTagseGFPExpressionMammalianMutationChanged Glycine 235 to AlaninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1501 - 1520 of 1875 results