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Plasmid#222462PurposeLevel 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-miniDFR (Solanum lycopersicum)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCHW101008
Plasmid#222453PurposeLevel 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.DepositorInsertCBS4-miniDFR::OsL5::NOS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111101
Plasmid#222452PurposeLevel 1, Position 7 containing CBS4-miniDFR::Cre::NOS.DepositorInsertCBS4-miniDFR::Cre::NOS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91003
Plasmid#222449PurposeLevel 1, Position 3 containing 35S::RUBY::OCS.DepositorInsert35S::RUBY::OCS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91070
Plasmid#222463PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber Mosaic Virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00014
Plasmid#222445PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.DepositorInsertVP16-NLS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110034
Plasmid#222443PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry.DepositorInsertLoxP-mCherry
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2142 MTK3-sfGFP
Plasmid#226661PurposeMTK3 part containing the coding sequence of sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternoneAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM034_P7_3"Lys3
Plasmid#216459Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''lys3
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM038_P8b_5'Lys3
Plasmid#216463Purpose5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.DepositorInsert5'lys3
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2368 MTK PB puro backbone
Plasmid#226673PurposeEmpty MTK PB cargo backbone marked with puromycin resistance.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
BRD4dN-mCherry-SspB
Plasmid#227387PurposeCondensate module, light-inducible oligomerization IDRDepositorInsertBRD4 IDR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-mCh-sspB
Plasmid#227377PurposeCondensate module, light-inducible oligomerization IDRDepositorInsertFUS IDR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB67
Plasmid#226316PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with K to A CsoS2 MR1-6
UseTagsExpressionBacterialMutationK261A, K320A, K380A, K436A, K495A, K546APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB74
Plasmid#226314PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-5
UseTagsExpressionBacterialMutationY300A, Y359A, Y420A, Y475A, Y534APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB73
Plasmid#226313PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-3
UseTagsExpressionBacterialMutationY300A, Y359A, Y420APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB72
Plasmid#226312PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1
UseTagsExpressionBacterialMutationY300APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB45
Plasmid#226304PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-2
UseTagsExpressionBacterialMutationC292S, C310S, C351S, C369SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB44
Plasmid#226303PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S MR1
UseTagsExpressionBacterialMutationC292S, C310SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only