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  2. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

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  8. Shim_CytC_TP_GFP_pK7FWG2

    Plasmid
    #202646
    Purpose
    Determine where transit peptide targets GFP in N. benthamiana
    Depositor
    Daniel Sloan
    Article
    DeTar et al bioRxiv 2023.08.01.551541
    Insert
    N-terminal transit peptide from putative Cytochrome C
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 10, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202646-plasmid-map-sequence-id-403492

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  15. pRA1SEGFPTcIfm

    Plasmid
    #193777
    Purpose
    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PR promoter and PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193777-plasmid-map-sequence-id-389829

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  18. LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3

    Plasmid
    #192397
    Purpose
    To test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).
    Depositor
    Ryan Lister
    Article
    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038
    Insert
    35S::FRT-OCS-FRT::B3RT-OCS-B3RT::Turbo GFP
    Use
    Synthetic Biology
    Tags
    N7
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192397-plasmid-map-sequence-id-378296

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Showing: 1481 - 1500 of 23388 results