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Plasmid#125397PurposeCRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide3
Plasmid#118179PurposeCRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta PBM + FBM
Plasmid#202416PurposeExpression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutationDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationLacking DTHL, and H477/R479/S481>AAA mutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCGLH-luciferase
Plasmid#194979PurposepCGLH control plasmidDepositorInsertluciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSico-shHDAC2
Plasmid#194983Purposeconditional knock down HDAC2DepositorInsertshHDAC2
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
SGEP-shRLuc-802
Plasmid#188668Purposecontrol shRNADepositorInsertshRLuc
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PCNA
Plasmid#188686Purposecontrol sgRNADepositorInsertsgPCNA (PCNA Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme N53A N55A V57A
Plasmid#18353DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationN53A N55A V57APromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-hEAAT5
Plasmid#32817DepositorInsertEAAT5 (SLC1A7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn Barcode11
Plasmid#226185PurposeExpression mappingDepositorInsertSyn Barcode11
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
mKO2-CytERM-N-17
Plasmid#57868PurposeLocalization: Endoplasmic Reticulum, Excitation: 551, Emission: 565DepositorInsertCytERM
UseTagsmKO2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBACH2.2.0-gDNA
Plasmid#176375PurposeExpresses spCas9 and gRNA sequence targeting BACH2DepositorInsertBACH2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_AAVS1
Plasmid#141212PurposepDECKO_mCherry_AAVS1_186. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPOU6F1.1.0-gDNA
Plasmid#176377PurposeExpresses spCas9 and gRNA sequence targeting POU6F1DepositorInsertPOU6F1 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP_AAVS1_186
Plasmid#159091Purposetargeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pREP4xN_ccdB
Plasmid#79022PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
C33m
Plasmid#120968PurposeMoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAiiA degradase (Bennett, no ssra tag)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREP3xN_ccdB
Plasmid#79021PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-MAP4-C-10
Plasmid#54923PurposeLocalization: Microtubules, Excitation: 568, Emission: 592DepositorInsertMAP4
UseTagsmAppleExpressionMammalianMutationaa 640-1045 of MAP4PromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -