Addgene

We narrowed to 7,539 results for: Dos

Showing: 1481 - 1500 of 7539 results

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

K855A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0001

Plasmid

#117523

Purpose

Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-DE (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

D1135E

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0049

Plasmid

#117616

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pOEM1_pCMV:axFgf8-pPH:vsvged

Plasmid

#111159

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Nacu et al Nature. 2016 May 19;533(7603):407-10. do

Insert

axFgf8

Use

Baculoviral rescue shuttle vector

Tags

Expression

Insect and Mammalian

Mutation

wt

Promoter

CMV

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b_PfSSB_W166C

Plasmid

#103001

Purpose

The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB (C93A,W166C), used for obtaining a fluorescent adduct at the cysteine

Depositor

Martin Webb

Article

Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

Insert

PfSSB_W166C

Use

Tags

Expression

Bacterial

Mutation

C93A, W166C

Promoter

T7

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAK22

Plasmid

#101741

Purpose

encoding two copies of T. thermophilus SecG for expression of SecYEG complex

Depositor

Tomoya Tsukazaki

Article

Sugano et al PLoS One. 2017 Aug 18;12(8):e0183434. do

Insert

Thermus thermophilus HB8 SecG x 2

Use

Tags

NONE

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Ppj220(JBEI-13483)

Plasmid

#89786

Purpose

for expression of C-StrepII-3607

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

3607

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006820

Plasmid

#87921

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006826

Plasmid

#87923

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006834

Plasmid

#87920

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JPUB_006828

Plasmid

#87922

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN51 (L1L2)

Plasmid

#80108

Purpose

Late1(delta)-O3-33-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (L1L2)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN51 (R40A)

Plasmid

#80109

Purpose

Late1-O3-33-myc-PH(R40A)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (R40A)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN51 (A167R)

Plasmid

#80110

Purpose

Late1-O3-33(A167R)-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (A167R)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN11 (L1L2)

Plasmid

#80111

Purpose

Palm1-Late1(delta)-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11 (L1L2)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN24 (L1L2)

Plasmid

#80114

Purpose

PH-Late1(delta)-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-24 (L1L2)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN25 (L1L2)

Plasmid

#80115

Purpose

I3-01p-myc-Late1(delta)-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-25 (L1L2)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only