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pcDNA3.1(+)_Granulin 4+linker5

Plasmid

#176924

Purpose

express Granulin 4 with linker 5 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 4+linker5 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP-Calnexin-N-14

Plasmid

#56214

Purpose

Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Calnexin (CANX Human)

Use

Tags

mIFP

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
TFORF2935

Plasmid

#144411

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MAFK (MAFK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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mCardinal-Calnexin-N-14

Plasmid

#56154

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Calnexin (CANX Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pSUPER-retro-puro-Smad2

Plasmid

#89827

Purpose

Knockdown human Smad2 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD2 shRNA (SMAD2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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DYRK1B gRNA (BRDN0001149482)

Plasmid

#76623

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK1B gRNA (BRDN0001149418)

Plasmid

#76621

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2272

Plasmid

#111898

Purpose

ssAAV genome with Ple342 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple342 (TUBB3 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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DYRK1B gRNA (BRDN0001146749)

Plasmid

#76622

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AT2G45340 K10_pECIA2

Plasmid

#115023

Purpose

Bait vector AT2G45340 K10_pECIA2 should be used with prey vector AT2G45340 K10_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G45340 (AT2G45340 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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DYRK1B gRNA (BRDN0001149374)

Plasmid

#76624

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DYRK4 gRNA (BRDN0001149224)

Plasmid

#75521

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK4 (DYRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK4 gRNA (BRDN0001145834)

Plasmid

#75519

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK4 (DYRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DYRK4 gRNA (BRDN0001147655)

Plasmid

#75520

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK4 (DYRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AT2G45340 K10_pECIA14

Plasmid

#114823

Purpose

Prey vector AT2G45340 K10_pECIA14 should be used with bait vector AT2G45340 K10_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G45340 (AT2G45340 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IRAK3 gRNA (BRDN0001146930)

Plasmid

#77714

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK3 (IRAK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IRAK3 gRNA (BRDN0001146977)

Plasmid

#77715

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK3 (IRAK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCSF-R(DD)-luc

Plasmid

#12424

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Dec . 14(12):8085-95

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

Deletion from bp-86 to -37 (created a KpnI site i…

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
MED9_pLX307

Plasmid

#98352

Purpose

Lentiviral expression of MED9

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MED9 (MED9 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1136

Plasmid

#29056

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple198 (SLC6A4 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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