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pBS ZfHoxb9a
Plasmid#27529DepositorInsertHoxb9a (hoxb9a Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
V21 CS2+ RFPbb
Plasmid#17112DepositorTypeEmpty backboneUseXenopus/mammalian/avian/zebrafishTagsRFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
V20 CS2+ RFPbe
Plasmid#17111DepositorTypeEmpty backboneUseXenopus/mammalian/avian/zebrafishTagsRFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA
Plasmid#74302PurposeExpression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafishDepositorUseZebrafishTagsFLAG, cmcl2::EGFP, and p2A-mCherryExpressionMutationTRPV4: delta 760-871PromoterNeurog1Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2iApowriter2(#336)
Plasmid#184104Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesisTagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2iU6Apowriter2N(#355)
Plasmid#184106Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesisDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesis + in vitro trans…Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-Ubi+SP6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Apowriter2(#340)
Plasmid#184105Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWtEos3N(#486)
Plasmid#184107Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWChe(#488)
Plasmid#184108Purposecyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-BioLHis
Plasmid#58949Purposebackbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tagDepositorTypeEmpty backboneUseTags6His tag and biotinylation peptideExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry
Plasmid#218860Purpose10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.DepositorInserthGCG-ICAM(1235)-nrxn1b
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pME-mTagBFP
Plasmid#75148PurposeTagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Mito-mRFP
Plasmid#169001PurposeExpress Mito (Su9)-mRFP in zebrafishDepositorInsertMito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
UseUnspecifiedTagsmRFPExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2pA2-mnx1:MLS-KillerRed
Plasmid#115515PurposePlasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neuronsDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoter-3mnx1Available sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk3a
Plasmid#208985PurposeRiboprobe generationDepositorInsertkcnk3a (kcnk3a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-MCS
Plasmid#75174PurposeMultiple cloning site for insertion of gene of interest for C-terminal fusions.DepositorInsertMultiple Cloning Site
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only
