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Plasmid#142181PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMLXIPL (MLXIPL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kir2.1Mut-T2A-tdTomato
Plasmid#60644PurposeExpresses Kir2.1Mut-T2A-tdTomatoDepositorInsertKcnj2 (Kcnj2 Mouse)
UseTagsmycExpressionMammalianMutationGYG to AAA (aa144-146)PromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-OSBP
Plasmid#134659PurposeFlag and GFP-tagged OSBPDepositorInsertOSBP (OSBP Human)
UseLentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GAL4-iLight-VP16
Plasmid#170274PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16
UseAAVTagsExpressionMutationSee CommentsPromoterCaMKIIAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-dTAG-Puro
Plasmid#207727PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Plasmid#100834PurposeAAV expression of ultrasensitive protein calcium sensor from CamKII promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCamKIIAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-2XFLAG-TMEM192
Plasmid#104435PurposeExpresses 2XFLAG-TMEM192 in mammalian cellsDepositorInsertTMEM-192 (TMEM192 Human)
UseLentiviralTags2XFLAGExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-m
Plasmid#136294Purposeexpresses ABE8.8-m in mammalian cellsDepositorInsertABE8.8-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only