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Showing: 1461 - 1480 of 1631 results

  1. pcDNA3-ICUE4

    Plasmid
    #181846
    Purpose
    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    ICUE4 (RAPGEF3 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181846-plasmid-map-sequence-id-364236

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  4. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2 (NES Synthetic)
    Use
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

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  5. Gs(short)-CASE

    Plasmid
    #168124
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG1
    Expression
    Mammalian
    Mutation
    NLuc is inserted at N136/V137 within GNAS
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168124-plasmid-map-sequence-id-332868

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  6. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  7. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid
    #156415
    Purpose
    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4A-G12D (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mTagBFP2
    Expression
    Mutation
    mutant KRAS4A-G12D
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156415-plasmid-map-sequence-id-308711

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  8. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

    Plasmid
    #156414
    Purpose
    kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4B-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Expression
    Mutation
    mutant KRAS4B-G12V
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156414-plasmid-map-sequence-id-308707

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  9. pDOE20 mC VN

    Plasmid
    #124246
    Purpose
    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in Agrobacterium- transformed plant cells.
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124246-plasmid-map-sequence-id-239323

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  10. pDOE20 mC VN transient

    Plasmid
    #124247
    Purpose
    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124247-plasmid-map-sequence-id-239326

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  11. Neuroligin-3 GFP/pCMV

    Plasmid
    #197338
    Purpose
    Plasmid expressing an antigen targeting Neuroligin-3
    Depositor
    Thomas Südhof
    Insert
    Neuroligin3 (Nlgn3 Mouse)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197338-plasmid-map-sequence-id-401573

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  12. pcDNA3-BG4-NLS-mVen

    Plasmid
    #211449
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Use
    Tags
    NLS-mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211449-plasmid-map-sequence-id-419262

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  13. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

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  14. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  15. Neuroligin-1-GFP/pCMV

    Plasmid
    #197340
    Purpose
    Plasmid expressing an antigen targeting Neuroligin-1
    Depositor
    Thomas Südhof
    Insert
    Neuroligin1 (Nlgn1 Mouse)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197340-plasmid-map-sequence-id-401589

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  18. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

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Showing: 1461 - 1480 of 1631 results