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Plasmid#216541PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2DepositorInsertPTK2 (S732A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S732APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS910A
Plasmid#216542PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2DepositorInsertPTK2 (S910A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S910APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-FRB-Cdc42/N12
Plasmid#214290PurposeExpress split-Cdc42 fragment fused with FRB and MBPDepositorInsertSplit-Cdc42 fragment fused with FRB (CDC42 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Cdc42/13C-FKBP-MBP
Plasmid#214291PurposeExpress split-Cdc42 fragment fused with FKBP and MBPDepositorInsertSplit-Cdc42 fragment fused with FKBP (CDC42 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationCdc42-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-FRB-RhoA/N12
Plasmid#214298PurposeExpress split-RhoA fragment fused with FRB and MBPDepositorInsertSplit-RhoA fragment fused with FRB (RHOA Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RhoA/13C-FKBP-MBP
Plasmid#214299PurposeExpress split-RhoA fragment fused with FKBP and MBPDepositorInsertSplit-RhoA fragment fused with FKBP (RHOA Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRhoA-Q63LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat Δ192Annexin A11
Plasmid#164498PurposeFor bacterial expression of rat His-tagged Δ192Annexin A11DepositorInsertrat Δ192Annexin A11 (Anxa11 Rat)
UseTagsHisExpressionBacterialMutationN-terminal truncationPromoterT7Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat Δ188Annexin A11
Plasmid#164497PurposeFor bacterial expression of rat His-tagged Δ188Annexin A11DepositorInsertrat Δ188Annexin A11 (Anxa11 Rat)
UseTagsHisExpressionBacterialMutationN-terminal truncationPromoterT7Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
Plasmid#162279PurposeEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN2A Variant 1, stabilized (SCN2A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp4551.PromoterCMVAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
Plasmid#61591PurposeA single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.DepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorInsertHero11 (C19orf53 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-FL
Plasmid#202515PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3172
Plasmid#144648PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSTAT6 (STAT6 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero13
Plasmid#187929PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero13DepositorInsertHero13 (BEX3 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRV-RB
Plasmid#106374Purposecreating stable line with RFP and BSD resistanceDepositorInsertmRFP and blasticidin resistance gene
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF3173
Plasmid#144649PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSTAT6 (STAT6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRV-GN
Plasmid#106373Purposecreating stable line with GFP and G418 resistanceDepositorInserteGFP and geneticin/G418 resistance gene
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS732A/S910A
Plasmid#221642PurposeThis retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2DepositorInsertPTK2 (S732A/S910A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK double mutation S732A/S910A (dominant negativ…PromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKP712/713A
Plasmid#216544PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2DepositorInsertPTK2 (P712/713A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation P712/713APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKR177/178A
Plasmid#216543PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2DepositorInsertPTK2 (R177/178A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation R177/178APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only