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Plasmid#103991PurposePlasmid encoding CloverDepositorInsertClover
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, ā¦PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Venus SX
Plasmid#103990PurposePlasmid encoding Venus SXDepositorInsertVenus SX
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, ā¦PromoterproCAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus JBC
Plasmid#103987PurposePlasmid encoding mVenus JBCDepositorInsertmVenus JBC
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, ā¦PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-EGFP-6H
Plasmid#187001PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Puro
Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "ā¦ExpressionMutationSilent V205V mutation in myogenin gene to preventā¦PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-mCherry-6H
Plasmid#187104PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVP65KR-gb-SacB(-)
Plasmid#171646PurposeEquipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.DepositorInsertMBP-mCherry-8xHis-SacB(-)
UseTags8xHisExpressionMutationinternal HindIII site in the SacB gene was removeā¦PromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZS*11-yfp0
Plasmid#53241Purposeexpress yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterPLtetO1Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDC2.1.0-gDNA
Plasmid#113781PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CSDC2DepositorInsertCSDC2 (CSDC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
GB4652
Plasmid#215266PurposeTU for the copper regulated expression of the flippase (FLP) recombinase.DepositorInsertCBS:miniDFR:FLP:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB1
Plasmid#204668PurposepUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructsDepositorInsertFRT-erm-FRT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E
Plasmid#125797PurposeHistone H2B-superfolder GFP and tdTomato fused by unskippable linkerDepositorInsertH2BsfGFP-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIJ8
Plasmid#68122PurposeTemperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expressionDepositorInsertsflp recombinase
rhaS
rhaR
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-VHL wt-pBabe-puro
Plasmid#19234DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwtPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Thy1.1 DEST
Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPIPL12-FnCas12a
Plasmid#199563PurposeTetracycline-Inducible Fncas12a gene expression in E. coli and clostridiaDepositorInsertFncas12a
UseCRISPR and Synthetic Biology; Tetracycline induciā¦TagsExpressionBacterialMutationPromoterCOtetR-PIPL12Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mCherry
Plasmid#99265PurposeContains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmCherry
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only