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Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
UseTagsNuclear localization signal (NLS)ExpressionYeastMutationPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+p35SoTMV
Plasmid#170876PurposePhytobrick - p35SoTMVDepositorInsertp35SoTMV
UseTagsExpressionPlantMutation214C>T and 229C>T to remove BbsI restrictio…PromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA13
Plasmid#158491PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationno STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
ACPPiso2-V5
Plasmid#113053PurposeExpresses human prostatic acid phosphatase (isoform 2) in mammalian cellsDepositorInsertprostatic acid phosphatase isoform 2 (ACP3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCoofy62
Plasmid#122014PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.242
Plasmid#73754PurposeNabi2.242 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.242
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-Linker_PCNA-MutC
Plasmid#98272Purposefor low level expression of mEos2-PCNA in mammalian cellsDepositorInsertPCNA (PCNA Human)
UseTagsmEos2ExpressionMammalianMutationSilent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…PromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB690
Plasmid#60134PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSG5-hSyntenin2b
Plasmid#89433Purposemammalian expressio of human Syntenin2bDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRKVSV FRNK
Plasmid#50534DepositorInsertprotein tyrosine kinase, domain
UseTags2X VSVExpressionMammalianMutationtruncated (NT2186-3268)PromoterCMVAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-hSyntenin2a
Plasmid#89421PurposeTwo-hybrid systemDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsGAL4 DBDExpressionYeastMutationPromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST td tomato Rab35 CA
Plasmid#58383Purposeexpression of drosophila tdtomato-Rab35 constitutively active mutantDepositorInsertRab35 (Rab35 Fly)
UseTagstd tomatoExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-hSyntenin2b
Plasmid#89422PurposeTwo-hybrid systemDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsGAL4 DBDExpressionYeastMutation* see belowPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB754
Plasmid#60157PurposeGreen beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterLYS9prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB691
Plasmid#60135PurposeRed beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab18 DN
Plasmid#58722Purposeexpression of drosophila YFP Rab18 dominant negative mutantDepositorInsertRab18 (Rab18 Fly)
UseTagsYFPExpressionInsectMutationS19NPromoterGAL4Available sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB753
Plasmid#60148PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterLEU1prAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRKVSV FAT
Plasmid#50533DepositorInsertprotein tyrosine kinase, domain
UseTags2X VSVExpressionMammalianMutationtruncated (NT2630-3268)PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-LAMP1-mGL
Plasmid#222394PurposeTo express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.DepositorInsertLAMP1 and mGreenlantern (LAMP1 Synthetic, Homo Sapiens, Human)
UseAAVTagsLAMP1 and mGreenLanternExpressionMammalianMutationPromoterCAG or CAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only