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Plasmid#211342PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211343PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211344PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3-[Δ1-32]ARL10-His6(CO)
Plasmid#201876PurposeUsed for bacterial expression of human protein ARL10DepositorInsertARL10 (ARL10 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationDeletion of residues 1-32 from N-terminus and cod…PromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-CD3D-274+5G>A
Plasmid#198023PurposeLV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in EcuadorDepositorInsertCD3D c.274+5G>A
UseLentiviralTagsExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF7b
Plasmid#169394PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFPDepositorInsertSARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 CEP164 WW domain
Plasmid#104276PurposeBacterial expression of WW domain from CEP164DepositorInsertCEP164 WW domain (CEP164 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 IQGAP2 WW domain
Plasmid#104280PurposeBacterial expression of WW domain from IQGAP2DepositorInsertIQGAP2 WW domain (IQGAP2 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ARHGAP9 WW domain
Plasmid#104286PurposeBacterial expression of WW domain from ARHGAP9DepositorInsertARHGAP9 WW domain (ARHGAP9 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 IQGAP3 WW domain
Plasmid#104289PurposeBacterial expression of WW domain from IQGAP3DepositorInsertIQGAP3 WW domain (IQGAP3 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 CEP164 WW domain
Plasmid#104300PurposeBacterial expression of WW domain from CEP164DepositorInsertCEP164 WW domain (CEP164 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 WWTR1 WW domain
Plasmid#104261PurposeBacterial expression of WW domain from WWTR1DepositorInsertWWTR1 WW domain (WWTR1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 WWOX WW domain #2
Plasmid#104236PurposeBacterial expression of WW domain #2 from WWOXDepositorInsertWWOX WW domain #2 (WWOX Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK021
Plasmid#75060PurposeProduces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)DepositorInsertphosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)
UseTagsExpressionBacterialMutationE. coli optimized codons in first 25 residuesPromoterlacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_λHA-GFPnonopt
Plasmid#210576PurposeExpresses lambdaHA-nonoptimal GFPDepositorInsertLambdaHA-GFP nonoptimal
UseTagslambda HAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Akaluc-N1
Plasmid#120371PurposeAkaluc luciferase mammalian expression in -N1 backboneDepositorInsertAkaluc (human optimized)
UseTagsExpressionMammalianMutationHuman codon optimizedPromoterCMVAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9D10A_GFP
Plasmid#44720PurposeCo-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9D10A-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationD10A mutation- nickase mutantPromoterCAGAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
UseTags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV301
Plasmid#204969PurposeAMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance markerDepositorInsertsMad7
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only