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Plasmid#190853PurposeFor mammalian expression of 3JB8F+4, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB8F_T1+4bp (A Dual-Specificity aptamer targeting OGT and β-catenin)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-3JB8R+12
Plasmid#190855PurposeFor mammalian expression of 3JB8R+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB8R_bc339+12bp (A Dual-Specificity aptamer targeting OGT and β-catenin)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
H1.99.luc
Plasmid#193663PurposeExpresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99)DepositorInsertTruncated Pol III H1 promoter (H1.99) + 41 nucleotides
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
H1.224.luc
Plasmid#193704PurposeExpresses a truncated PolII H1 promoter (H1.244)DepositorInsertTruncated PolIII H1 promoter of 224 nucleotides (H1.224) + N41 fragment
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
H1.224.M.luc
Plasmid#193705PurposeExpresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutatedDepositorInsertTruncated PolIII H1 promoter (H1.224) with the TATA box mutated
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA PSPC1 Y523F
Plasmid#124373PurposeExpresses PSPC1 Y523F in mammalian cellsDepositorInsertPSPC1 (PSPC1 Human)
UseTagsHA and HA tagExpressionMammalianMutationY523FPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14A)-mVenus
Plasmid#168499PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9R)-mVenus
Plasmid#168498PurposeMammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(K8,9A)-mPAGFP
Plasmid#168494PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertK8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14K)-mVenus
Plasmid#168488PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9E)-mVenus
Plasmid#168487PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K22,24A)-mVenus
Plasmid#168484PurposeMammalian expression of the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9A)-mVenus
Plasmid#168483PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (G2A)-mVenus
Plasmid#168482PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
sg2+sg3
Plasmid#113970PurposeDouble short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg2+sg3
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_3xFLAG
Plasmid#101653PurposeExpression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, 3xFLAG tag
UseTags3xFLAG tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC29
Plasmid#104803PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr4g094545
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta2
Plasmid#100871PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseTags3xFLAGExpressionMammalianMutationtruncated protein 71-246aaPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-Flag-NLS-hSPDEF delta3
Plasmid#100876PurposeLentiviral expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationtruncated protein 243-335aaPromoterTRE2Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta3
Plasmid#100872PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseTags3xFLAGExpressionMammalianMutationtruncated protein 244-335aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only