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Plasmid#124673PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3458)
Plasmid#160638PurposepEGB3a1 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X2-hDrp1(518-736)-Flag
Plasmid#37244DepositorInsertDrp1 (DNM1L Human)
UseTagsFLAG and GSTExpressionBacterialMutationcontains AA 517-endPromoterAvailable sinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
PLK3 1-334 T219D-pEGFP
Plasmid#23268DepositorInsertPLK3 (PLK3 Human)
UseTagspEGFPExpressionMammalianMutationaa 1-334 T219DPromoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
XE120 CAMKII-K42M 1-271-CS2+
Plasmid#16734DepositorInsertCAMKII-K42M 1-271 (Camk2a Rat)
UseXenopus expressionTagsExpressionMutationC-terminal truncation of K42M kinasePromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-1xPerfect
Plasmid#124674PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCY5.1nh
Plasmid#160299PurposeYeast CRISPR plasmid targeting the natMX and hphMX cassettesDepositorInsertssgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3MX selection cassette
sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
Cas9
UseCRISPRTags8xHis-tag and SV40 NLSExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCY5.1kn
Plasmid#160298PurposeYeast CRISPR plasmid targeting the kanMX and natMX cassettesDepositorInsertssgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
URA3MX selection cassette
sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
Cas9
UseCRISPRTags8xHis-tag and SV40 NLSExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4QE2Crimson
Plasmid#218280PurposeIntroduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoterDepositorInsertpURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q70P/Q443P
Plasmid#216403PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q70P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 70, 443 to P…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
Plasmid#216404PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56, Glutamine 70, 443 and Asp…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG
Plasmid#212809PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cellsDepositorInsertE3(63)
UseTagsNUbo and mCherry-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7
Plasmid#212804PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cellsDepositorInsertNUbo-E3(48)-P2A-3xFLAG-Ubc7
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG
Plasmid#212803PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cellsDepositorInsertE3(48)
UseTagsNUbo and mCherry-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-NLS-VSV
Plasmid#212746PurposeDoxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-NLS-VSV
Plasmid#212756PurposeDoxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo
Plasmid#212800PurposeConstitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cellsDepositorInsertE3(1)
UseTagsCUbo and MycExpressionMammalianMutationHOIP(C885A)PromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)
Plasmid#212814PurposeConstitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cellsDepositorInsertH2B
UseTags8xHis and CUbo(K63R)ExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-mCherry-FLAG
Plasmid#212798PurposeConstitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cellsDepositorInsertNUbo
UseTagsmCherry-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His7-NUbo-GFP
Plasmid#212791PurposeCopper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeastDepositorInsertHis7-NUbo-GFP
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only