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pHR-iCasp9

Plasmid

#199441

Purpose

Express inducible Caspase9 in mammalian cells

Depositor

Chao Tang

Article

Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. do

Insert

inducible Caspase9 (CASP9 Human)

Use

Lentiviral

Tags

DmrB and mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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hCathepsin L

Plasmid

#11250

Purpose

Mammalian expression of human cathepsin L

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2005 Dec 8. ():.

Has Service

Cloning Grade DNA

Insert

cathepsin L (CTSL Human)

Use

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f

Plasmid

#162373

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Use

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

CMV

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro

Plasmid

#8453

Purpose

3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

Depositor

Bob Weinberg

Article

Stewart et al RNA 2003 Apr;9(4):493-501.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

Plasmid

#153003

Purpose

Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C) (G74)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-smURFP

Plasmid

#80349

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Insert

smURFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1

Plasmid

#52326

Purpose

expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Use

Tags

Expression

Mammalian

Mutation

R117Q mutation (R95Q according to numbering in Fi…

Promoter

CMV

Available since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pN1/G3BP1-iRFP

Plasmid

#129339

Purpose

Stress granule marker

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

iRFP (from Dr. M. Matsuda)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-puro

Plasmid

#1764

Purpose

Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.

Depositor

Jay Morgenstern, Bob Weinberg, Hartmut Land

Article

Morgenstern et al Nucleic Acids Res. 1990 Jun 25;18(12):35

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2005

Availability

Academic Institutions and Nonprofits only

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