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Showing: 1441 - 1460 of 49625 results

  1. hSyn-DIO-somBiPOLES-eGFP

    Plasmid
    #192584
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192584-plasmid-map-sequence-id-379290

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  2. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid
    #195487
    Purpose
    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.
    Depositor
    HHMI-JRC Tool Translation Team, Tim Brown
    Insert
    HaloTag.v7
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    C61T, C262V
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195487-plasmid-map-sequence-id-393190

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  3. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid
    #177209
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177209-plasmid-map-sequence-id-348605

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  4. HCN3/pCDNA3.1

    Plasmid
    #197293
    Purpose
    Plasmid expressing an antigen targeting the HCN3 ion channel subunit
    Depositor
    Dane Chetkovich
    Insert
    HCN3 (Hcn3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197293-plasmid-map-sequence-id-388075

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  5. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

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  6. pKM510

    Plasmid
    #134227
    Purpose
    Retroviral plasmid expressing mCherry - HJURP
    Depositor
    Iain Cheeseman
    Insert
    mCherry-HJURP-LacI 1-304 (HJURP Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    134227-plasmid-map-sequence-id-271958

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  7. pSBtet-RB-PE2

    Plasmid
    #173903
    Purpose
    SB-transposon with inducible expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173903-plasmid-map-sequence-id-346343

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  8. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

    Plasmid
    #128174
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128174-plasmid-map-sequence-id-247966

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  9. pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

    Plasmid
    #128171
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128171-plasmid-map-sequence-id-247962

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  10. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid
    #100050
    Purpose
    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    hChR2(E123T/H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized, E123T/H134R
    Promoter
    CaMKIIa
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100050-plasmid-map-sequence-id-194451

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  11. pDEST26-his-hSFRP1

    Plasmid
    #189539
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    his
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189539-plasmid-map-sequence-id-374149

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  12. pZBTB5-donor

    Plasmid
    #112303
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112303-plasmid-map-sequence-id-228415

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  13. pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid
    #100049
    Purpose
    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    hChR2(E123T/H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized, E123T/H134R
    Promoter
    hSynap
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100049-plasmid-map-sequence-id-194448

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  14. pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

    Plasmid
    #32521
    Depositor
    Mustafa Sahin
    Insert
    Dominant negative Rheb (D60I) (Rheb Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D60I to make dominant negative
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. pBS ZfHoxb1a

    Plasmid
    #27519
    Depositor
    Victoria Prince
    Insert
    Hoxb1a (hoxb1a Zebrafish)
    Use
    Riboprobe synthesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-CamKIIa-C1V1::FusionRed::Kv2.1

    Plasmid
    #102771
    Purpose
    "Activate neurons with 2p stimulation"
    Depositor
    Karel Svoboda
    Insert
    C1V1::FusionRed::Kv2.1
    Use
    AAV
    Tags
    FusionRed and Kv2.1 traficking domain
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102771-plasmid-map-sequence-id-239474

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  17. SPIN3A

    Plasmid
    #74656
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPIN3A (SPIN3 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid
    #137859
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137859-plasmid-map-sequence-id-274442

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  19. pZFX-donor

    Plasmid
    #112366
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFX with GFP
    Depositor
    Kevin White
    Insert
    ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112366-plasmid-map-sequence-id-229714

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  20. pBS ZfHoxa2b

    Plasmid
    #27514
    Depositor
    Victoria Prince
    Insert
    Hoxa2b (hoxa2b Zebrafish)
    Use
    Riboprobe synthesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 7, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 1441 - 1460 of 49625 results