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Showing: 1441 - 1460 of 18103 results

  4. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid
    #90011
    Purpose
    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e
    Depositor
    Julien Béthune
    Article
    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.
    Inserts
    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    Use
    Retroviral; Flp/frt
    Tags
    FLAG and Myc
    Expression
    Mammalian
    Mutation
    Ser 216 to Ala
    Promoter
    CMV
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    90011-plasmid-map-sequence-id-201243

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  5. TLNRD1(4H)-2T_pET47b

    Plasmid
    #224069
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    L191T/A225T double mutant; reduces affinity of TL…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224069-plasmid-map-sequence-id-443086

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  6. TLNRD1(4H)-2E_pET151

    Plasmid
    #224068
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS; V5
    Expression
    Bacterial
    Mutation
    K192E/R233E double mutant; prevents actin binding…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224068-plasmid-map-sequence-id-443085

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  7. CCM2_I432D_eGFP

    Plasmid
    #208879
    Purpose
    Express eGFP-CCM2 with the I432D mutation in mammalian cells.
    Depositor
    Guillaume Jacquemet
    Article
    Neil J. et al BioRxiv
    Insert
    CCM2 (CCM2 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    mutation I432D
    Promoter
    Available since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208879-plasmid-map-sequence-id-416571

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  8. CCM2_W421AD422A_eGFP

    Plasmid
    #208880
    Purpose
    Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.
    Depositor
    Guillaume Jacquemet
    Article
    Neil J. et al BioRxiv
    Insert
    CCM2 (CCM2 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    mutations W421A and D422A
    Promoter
    Available since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208880-plasmid-map-sequence-id-416577

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  10. CCM2_I428S_eGFP

    Plasmid
    #208878
    Purpose
    Express eGFP-CCM2 with the I428S mutation in mammalian cells.
    Depositor
    Guillaume Jacquemet
    Article
    Neil J. et al BioRxiv
    Insert
    CCM2 (CCM2 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    mutation I428S
    Promoter
    Available since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208878-plasmid-map-sequence-id-416572

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  20. EGFP-CAAX

    Plasmid
    #86056
    Purpose
    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Homo sapiens Harvey rat sarcoma (HRAS Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains the region from 825-857 of HRAS as a far…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86056-plasmid-map-sequence-id-177924

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Showing: 1441 - 1460 of 18103 results