Addgene

We narrowed to 4,874 results for: hwa

Showing: 1441 - 1460 of 4874 results

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Mikko Taipale, Susan Lindquist

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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N-WASP

Plasmid

#186132

Purpose

For expression of recombinant N-WASp in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

WASL (WASL Rat)

Use

Tags

His6

Expression

Bacterial

Mutation

Amino Acid residues 183-193 fused to 273-501

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG

Plasmid

#139961

Purpose

Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET23-PBD(65-150)-N-Cys

Plasmid

#13722

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD(65-150)-N-Cys (PAK1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Met-Cys-Gly-PBD(65-150)-

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
pCMVHA hEZH2

Plasmid

#24230

Purpose

Mammalian expression of human EZH2 with HA tag

Depositor

Kristian Helin

Article

Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

Insert

EZH2 (EZH2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2B-GFP

Plasmid

#116869

Purpose

Nuclear localized GFP

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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NLRP3-mNeonGreen

Plasmid

#200942

Purpose

NLRP3 fused with mNeonGreen to indicate its localization change

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

Plasmid

#71761

Purpose

mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

Depositor

Liqun Luo

Article

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Inserts

mGFP
Synaptophysin

Use

AAV

Tags

Palmitoylation signal and mRuby

Expression

Mutation

Promoter

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
EcSTH

Plasmid

#211923

Purpose

This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

EcSTH

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Use

Tags

Expression

Bacterial

Mutation

E36K

Promoter

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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hTLR3-pcDNA3

Plasmid

#32712

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

TLR3 (TLR3 Human)

Use

Tags

FLAG and Preprototrypsin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2

Plasmid

#14129

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 (TSC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pSUPER IKKe

Plasmid

#26211

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK epsilon (IKBKE Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
mATF4

Plasmid

#24874

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mouse ATF4 (Atf4 Mouse)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
pBAD-Lime

Plasmid

#191255

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein Lime

Depositor

Robert Campbell

Article

Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

Insert

Lime

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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myc-mTOR

Plasmid

#1861

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

mTOR (Mtor Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher

Plasmid

#35044

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher

Use

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Promoter

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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Alpha1

Plasmid

#118044

Purpose

GoldenBraid2.0 Alpha 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pELuc

Plasmid

#118056

Purpose

GBPart - ELuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

ELuc

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Omega 1

Plasmid

#118046

Purpose

GoldenBraid2.0 Omega 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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