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Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i5 sgRNA / hSpCas9
Plasmid#172829PurposeMammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i3 sgRNA / hSpCas9
Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0193_Cq-Rpl40-Cas9
Plasmid#169346PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0193_Cq-Rpl40-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ002413Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9H(-10TC)_arr2
Plasmid#149587Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-eCas9-D1135E
Plasmid#154926PurposeaTc inducible eCas9 with D1135E mutationDepositorInserteCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationK848A, K1003A, R1060A, D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-OptiCas9-D1135E
Plasmid#154928PurposeaTc inducible OptiCas9 with D1135E mutationDepositorInsertOptiCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationR661A, K1003H, D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-HypaCas9-D1135E
Plasmid#154930PurposeaTc inducible HypaCas9 with D1135E mutationDepositorInsertHypaCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN692A/M694A/Q695A/H698A/D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9-D1135E
Plasmid#154931PurposeaTc inducible Cas9 with D1135E mutationDepositorInsertCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationD1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-D1135E
Plasmid#154924PurposeaTc inducible Cas9HF1 with D1135E mutationDepositorInsertCas9HF1-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN497A/R661A/Q695A/Q926A/D1135E and stop codon on …PromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-NG-nCas9
Plasmid#163539PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-nCas9
Plasmid#163538PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31APromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9
Plasmid#163564PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_arr2
Plasmid#149582Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9G(-10TC)_arr2
Plasmid#149585Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9B(-10TC)_arr2
Plasmid#149586Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_arr2
Plasmid#149575Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9B(RBSmut)_arr2
Plasmid#149579Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only