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Plasmid#22772DepositorInsertChR2-tdimer2RFP
UseTagsChR2-tdimer2RFPExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP L5-L2
Plasmid#62157PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJM100: no promoter RFP on pCM66T
Plasmid#74739PurposepCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoternoneAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-control -- AEB3mRFP1
Plasmid#63851PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAEB3Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-miRFP703_PGK-Puro
Plasmid#216667PurposeExpresses tTa-inducible miRFP703, with puromycin resistanceDepositorInsertsmiRFP703
PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H3C2
Plasmid#227335PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)DepositorArticleInsertH3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H2BC11
Plasmid#227331PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorArticleInsertH2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-9YS-miRFP670-TRF1
Plasmid#227381PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 9YS (TERF1 Human)
UseLentiviralTagsExpressionMutation9YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-27YS-miRFP670-TRF1
Plasmid#227383PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 27YS (TERF1 Human)
UseLentiviralTagsExpressionMutation27YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-15YS-miRFP670-TRF1
Plasmid#227382PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 15YS (TERF1 Human)
UseLentiviralTagsExpressionMutation15YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-5YS-miRFP670-TRF1
Plasmid#227380PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 5YS (TERF1 Human)
UseLentiviralTagsExpressionMutation5YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-3YS-miRFP670-TRF1
Plasmid#227379PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 3YS (TERF1 Human)
UseLentiviralTagsExpressionMutation3YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-miRFP(-5)-HO1
Plasmid#219424PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorArticleInsertsmiRFP682
HO1
UseTagsMGHHHHHGGExpressionBacterialMutationContains Ribosomal Binding Site for expression of…PromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)
Plasmid#224490PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-iRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)
Plasmid#224491PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-tdiRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 ymiRFP680-T2A-mTurquoise2
Plasmid#219840Purposeequimolar expression of ymiRFP680 and mTurquoise2 in budding yeastDepositorInsertymiRFP680-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF1 yemiRFP670-T2A-mTurquoise2
Plasmid#219841Purposeequimolar expression of yemiRFP670 and mTurquoise2 in budding yeastDepositorInsertyemiRFP670-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-ArgiNLS-miRFP670
Plasmid#220600PurposeConstitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.DepositorInsertmiRFP670
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKSR1-CA3-mRFP1 (C-KSR)
Plasmid#217754PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseTagsmRFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKSR1-CA3-GS-mRFP1 (C-KSR-GS)
Plasmid#217758PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseTagsmRFPExpressionMammalianMutationCA3 domain aa 317-400 with GGSSGGGGA linkerPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only