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Plasmid#55124PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab11a (RAB11A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB80-hKif5b(1-560)rigor-2xmNeongreen
Plasmid#174649PurposeLive-cell marker for stable microtubulesDepositorInserthKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
UseTagsmNeongreen-mNeongreenExpressionMammalianMutationrigor mutation G234APromoterChicken beta-actinAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
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HO-hisG-URA3-hisG-poly-HO
Plasmid#51663Purposeyeast plasmid for integration of target sequence at HO locus, URA3 selectionDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV/L7-6-GFP-WPRE
Plasmid#126462PurposeAn AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.DepositorInsertL7-6, Purkinje cell specific promoter
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChrimsonR-tdT
Plasmid#59171PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS598 EF1alpha-EGFPcre
Plasmid#11923DepositorInsertEGFP-cre (cre bacteriophage P1)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti puro HA-Ubiquitin
Plasmid#74218PurposeLentiviral expression of HA tagged UbiquitinDepositorInsertubiquitin (UBB Human)
UseLentiviralTagsHAExpressionMammalianMutationSingle ubiquitin coding sequence (full length UBB…PromoterCMVAvailable sinceMarch 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330a dCas9-KRAB
Plasmid#92361PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ubiquitin
Plasmid#187910PurposeMammalian cell expression of EGFP-tagged UbiquitinDepositorInsertUBB (UBB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…PromoterAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA
Plasmid#128034PurposeMammalian expression vector with N terminal HA tagDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSXneo 270(T2AG3)
Plasmid#12403DepositorInsert270(T2AG3)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
p27-IRES-mCherry
Plasmid#80143Purposeretroviral expression of p27 and mCherryDepositorInsertp27 (CDKN1B Human)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCherry
Plasmid#79130PurposeLentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherryDepositorInserttBFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6d:sgRNA#5
Plasmid#64249Purposeexpresses sgRNA under U6d promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZsGreen1-N1
Plasmid#54702PurposeLocalization: N1 Cloning Vector, Excitation: 493, Emission: 505DepositorTypeEmpty backboneUseTagsZsGreen1ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max_P2A_GFP
Plasmid#112099PurposeC:G-to-T:A base editingDepositorInsertBE4max_P2A_GFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only