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Plasmid#71878PurposeFor mitochondrial unfolded protein response researchDepositorInsertOTC-delta (Otc Rat)
UseTagsExpressionMutationDeleted amino acid 30-114 from full length OTCPromoterAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B2.0
Plasmid#99888PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
c-myc-PT3EF1a
Plasmid#92046PurposeExpresses c-Myc in mammalian cellsDepositorInsertC-MYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceJuly 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV_Cas9_puro
Plasmid#65655PurposeRetroviral introduction of Cas9 into mammalian cell line.DepositorInsertCas9
UseCRISPR and RetroviralTags5 ' FLAG tagExpressionMammalianMutationPromoterMSSV_LTRAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pVEX-CC1
Plasmid#74170Purposeprotein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteriaDepositorInsertDCTN1 (DCTN1 Chicken)
UseTagsNoneExpressionBacterialMutationPromoterInducible Tac promoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tagger
Plasmid#129396PurposeExpresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoterDepositorInsertrpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
UseTagsFLAG, HA, and V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-hGSDME
Plasmid#154876PurposeExpresses hGSDME in mammalian cellsDepositorInsertGSDME (GSDME Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)
Plasmid#128164PurposeUV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only