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pcDNA3.1-2xFLAG-2xSTREP-CDK4

Plasmid

#172615

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK4 (CDK4 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-5xA

Plasmid

#92007

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RIαΔ(D/D+linker)-EGFP

Plasmid

#181843

Purpose

GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of residues 12-112 in PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-KLF12/pcDNA3.1-Neo

Plasmid

#81069

Purpose

Expression of epitope-tagged human KLF12 in mammalian cells.

Depositor

Julian Downward

Article

Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

Insert

FLAG-KLF12 (KLF12 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαq-124-NLuc

Plasmid

#207153

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAQ (GNAQ Human)

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position F(124)E

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

Plasmid

#207160

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNG2 (GNG2 Human)

Use

Tags

Expression

Mammalian

Mutation

N-terminally tagged with LSS-mKATE2

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR-Kras

Plasmid

#118408

Purpose

Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-Kras

Use

Tags

C-terminal targeting sequence from Kras

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-2AFP-KDEL

Plasmid

#154349

Purpose

Mammalian expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + codon optimized Brn3a

Plasmid

#86829

Purpose

mammalian expression of the transcription factor Brn3a

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

Brn3a (POU4F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RasAR(R89L)-NES

Plasmid

#205568

Purpose

RasAR negative-control mutant plasmid. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR(R89L)-NES (RAF1 Human, Synthetic)

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Arg 89 mutated to Leu in Raf1 RBD region.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

Plasmid

#112840

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Full length Grb2

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-kappa-HA-dL5-BKa

Plasmid

#73212

Purpose

Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

Insert

BKalpha (Kcnma1 Mouse)

Use

Tags

dL5**

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

Plasmid

#112829

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Insert

LL5BIP (2410002F23Rik Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a WT pcDNA3.1 Zeo (+)

Plasmid

#206079

Purpose

Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro-Mfn2-ActA

Plasmid

#191981

Purpose

Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

Depositor

Luca Scorrano

Insert

Mfn2-ActA

Use

Tags

ActA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-delta2-26-FLAG

Plasmid

#35983

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Use

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1 aa 2-26 deleted

Promoter

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Dicer-delHelicC

Plasmid

#51366

Depositor

Narry Kim

Article

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to HelicC domain deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS8/myc-His

Plasmid

#127489

Purpose

Expresses myc-tagged NDUFS8 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS8 (NDUFS8 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

Plasmid

#127322

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-5xE

Plasmid

#92008

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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