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Showing: 1421 - 1440 of 1538 results

  2. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

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  3. TDP-43 mRuby K84ONBK

    Plasmid
    #216226
    Purpose
    TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.
    Depositor
    Jennifer Lee
    Article
    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do
    Insert
    Transactive response DNA binding protein of 43 kDa (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lysine 84 mutated to a TAG stop codon
    Promoter
    CMV Promoter
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216226-plasmid-map-sequence-id-431056

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  8. pJCC_162 SpCas9 KES(1107-1109)GG

    Plasmid
    #179526
    Purpose
    For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 KES(1107-1109)GG
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    replaced KES (residues 1107-1109) with GG
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179526-plasmid-map-sequence-id-351748

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  9. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid
    #179528
    Purpose
    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 R1333A/R1335A
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed arginines 1333 and 1335 to alanine
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179528-plasmid-map-sequence-id-351744

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  16. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

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  17. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

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Showing: 1421 - 1440 of 1538 results