Skip to main content
Addgene
Showing: 1421 - 1440 of 16182 results

  3. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Expression
    Mutation
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

    Enlarge

  4. AAV2_CAG_oROS-HT(C199S)_WPRE

    Plasmid
    #216415
    Purpose
    Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    oROS-HT(C199S)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216415-plasmid-map-sequence-id-429726

    Enlarge

  6. pRS140

    Plasmid
    #197141
    Purpose
    scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EGFP(M1V)
    Promoter
    CMV
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197141-plasmid-map-sequence-id-392024

    Enlarge

  7. pRS191

    Plasmid
    #197140
    Purpose
    scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EGFP(M1V)
    Promoter
    EF1a
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197140-plasmid-map-sequence-id-392613

    Enlarge

  8. pNM1325

    Plasmid
    #197144
    Purpose
    CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Insert
    SpCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    SpCas9(D10A)
    Promoter
    CAG
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197144-plasmid-map-sequence-id-392604

    Enlarge

  11. pLV-CS-117

    Plasmid
    #197134
    Purpose
    CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EGFP(M1V)
    Promoter
    CMV
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197134-plasmid-map-sequence-id-392599

    Enlarge

  12. pSI-218

    Plasmid
    #197139
    Purpose
    All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    SpCas9-PmCDA1-UGI
    Expression
    Mammalian
    Mutation
    SpCas9(D10A)
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197139-plasmid-map-sequence-id-392598

    Enlarge

  13. pLV-CS-060

    Plasmid
    #197133
    Purpose
    CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    MCP(N55K)
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197133-plasmid-map-sequence-id-392596

    Enlarge

  14. pLV-CS-193

    Plasmid
    #197137
    Purpose
    CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    mCherry(M1V/M9A)
    Promoter
    CMV
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    197137-plasmid-map-sequence-id-391932

    Enlarge

  15. pLV-CS-119

    Plasmid
    #197135
    Purpose
    CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EGFP(M1V)
    Promoter
    CMV
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    197135-plasmid-map-sequence-id-391931

    Enlarge

  17. LSB-hsa-miR-27a-5p

    Plasmid
    #103382
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-27a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-27a-5p target (MIR27A Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    103382-plasmid-map-sequence-id-195813

    Enlarge

  18. LSB-hsa-miR-30c-2-3p

    Plasmid
    #103414
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-30c-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-30c-2-3p target (MIR30C2 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    103414-plasmid-map-sequence-id-195877

    Enlarge

  19. LSB-hsa-miR-624-5p

    Plasmid
    #103681
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-624-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-624-5p target (MIR624 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    103681-plasmid-map-sequence-id-196410

    Enlarge

  20. LSB-hsa-miR-942-3p

    Plasmid
    #103758
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-942-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-942-3p target (MIR942 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103758-plasmid-map-sequence-id-196564

    Enlarge

Showing: 1421 - 1440 of 16182 results