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pT7-RiboJ-LacZa-T7

Plasmid

#173224

Purpose

pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-DNPH1

Plasmid

#203224

Purpose

For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

Depositor

Vern Schramm

Article

Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

Insert

DNPH1 (DNPH1 Human)

Use

Tags

His tag (with TEV cleavage site)

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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AA019

Plasmid

#216039

Purpose

Fragmid fragment: (C' terminus) VP64; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

VP64_v2.1; NpNLS_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-A-ccdB-G-Z

Plasmid

#218892

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-W-A-ccdB-G-X

Plasmid

#218891

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA335

Plasmid

#216069

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Stop; IRES2_v1; Start; HygroR_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AA294

Plasmid

#215968

Purpose

Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U1a_v1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA018

Plasmid

#215976

Purpose

Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; VP64_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA177

Plasmid

#215983

Purpose

Fragmid fragment: (N' terminus) Start codon only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA134

Plasmid

#216044

Purpose

Fragmid fragment: (C' terminus) SV40NLS; Linker; MeCP2

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

SV40NLS_v1.7; Linker_v4.1; MeCP2_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA006

Plasmid

#216056

Purpose

Fragmid fragment: (2A Selection) Blasticidin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

T2A_v1.1; BlastR_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA436

Plasmid

#216035

Purpose

Fragmid fragment: (Cas protein) binds MS2 stem loops

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

MS2_v1.1

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA440

Plasmid

#216036

Purpose

Fragmid fragment: (Cas protein) binds to TRE in absence of doxycycline

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

TetR_v1.1

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA199

Plasmid

#216050

Purpose

Fragmid fragment: (C' terminus) p65; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

p65_v1.1; NpNLS_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA266

Plasmid

#216064

Purpose

Fragmid fragment: (2A Selection) TagRFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

P2A_v1.1; TagRFP_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA201

Plasmid

#216026

Purpose

Fragmid fragment: (Cas protein) firefly luciferase

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Fluc_v1.1

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA267

Plasmid

#216065

Purpose

Fragmid fragment: (2A Selection) TagRFP657

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

P2A_v1.1; TagRFP657_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA333

Plasmid

#216067

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; PuroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Stop; IRES2_v1; Start; PuroR_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA200

Plasmid

#216051

Purpose

Fragmid fragment: (C' terminus) p65; HSF1; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

p65_v1.2; HSF1_v1.1; NpNLS_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge