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Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJQR3
Plasmid#218289PurposeIntroduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6DepositorInsertHO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036WUseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEdv1
Plasmid#218283PurposeUse together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locusDepositorInsertHph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEu2
Plasmid#218282PurposeUse together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locusDepositorInsertHO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJE13HD7
Plasmid#218281PurposeUse together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.DepositorInsertHO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA294
Plasmid#215968PurposeFragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoterDepositorHas ServiceCloning Grade DNAInsertU1a_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA018
Plasmid#215976PurposeFragmid fragment: (N' terminus) 4xVP16 transcritional activatorsDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; VP64_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA020
Plasmid#216004PurposeFragmid fragment: (Cas protein) binds PP7 stem loopsDepositorInsertPP7 Core Protein (PCP)_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA366
Plasmid#215970PurposeFragmid fragment: (Pol 2 promoter) Gal4-induced expressionDepositorHas ServiceCloning Grade DNAInsert5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA436
Plasmid#216035PurposeFragmid fragment: (Cas protein) binds MS2 stem loopsDepositorInsertMS2_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA440
Plasmid#216036PurposeFragmid fragment: (Cas protein) binds to TRE in absence of doxycyclineDepositorInsertTetR_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA201
Plasmid#216026PurposeFragmid fragment: (Cas protein) firefly luciferaseDepositorInsertFluc_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA299
Plasmid#216029PurposeFragmid fragment: (Cas protein) NNGG PAMDepositorInsertCas9_v1.1 [Slug]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA296
Plasmid#216028PurposeFragmid fragment: (Cas protein) NNNNCC PAMDepositorInsertNme2Cas9_v1.1 [Nme2]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA184
Plasmid#216047PurposeFragmid fragment: (C' terminus) for prime editing; using PEmaxDepositorInsertSV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA033
Plasmid#216013PurposeFragmid fragment: (Cas protein) deactivated CasDepositorInsertdCas9-NG_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA333
Plasmid#216067PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; PuroR; StopDepositorInsertStop; IRES2_v1; Start; PuroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA180
Plasmid#216023PurposeFragmid fragment: (Cas protein) NGRR(T) PAMDepositorInsertCas9_v1.1 [Sa]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA271
Plasmid#216074PurposeFragmid fragment: (CROPseq) guide expression; UMI-compatibleDepositorInsertWPRE_v1; U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]; 3'LTR; bGHpolyA_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA414
Plasmid#215991PurposeFragmid fragment: (N' terminus) conditional degradationDepositorHas ServiceCloning Grade DNAInsertStart; V5_v1.1; HaloTag_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA156
Plasmid#216022PurposeFragmid fragment: (Cas protein) Nanobody binds ALFA tagDepositorInsertNbALFA_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA391
Plasmid#216033PurposeFragmid fragment: (Cas protein) reverse TetR; binds to TRE in presence of doxycyclineDepositorInsertrTetR_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA349
Plasmid#216032PurposeFragmid fragment: (Cas protein) Renilla luciferaseDepositorInsertRluc_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA035
Plasmid#216015PurposeFragmid fragment: (Cas protein) deactivated CasDepositorInsertdCas9-SpRY_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA185
Plasmid#216048PurposeFragmid fragment: (C' terminus) for prime editing; using PEmax; for PE4 & 5DepositorInsertSV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1; P2A_v1.2; MLH1dn_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA008
Plasmid#216003PurposeFragmid fragment: (Cas protein) preferred over wildtype Cas12aDepositorInsertCas12a_v1.1 [EnAs]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA034
Plasmid#216014PurposeFragmid fragment: (Cas protein) deactivated CasDepositorInsertdCas9-SpG_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA301
Plasmid#216030PurposeFragmid fragment: (Cas protein) NNGG PAMDepositorInsertCas9_v1.1 [Sauri]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA367
Plasmid#215971PurposeFragmid fragment: (Pol 2 promoter) Copper-induced expressionDepositorHas ServiceCloning Grade DNAInsertMT_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA058
Plasmid#216040PurposeFragmid fragment: (C' terminus) for prime editingDepositorInsertMMLV-RT_v1.1; SV40NLS_v1.3
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA413
Plasmid#215990PurposeFragmid fragment: (N' terminus) conditional degradationDepositorHas ServiceCloning Grade DNAInsertStart; V5_v1.1; dTAG_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA226
Plasmid#216027PurposeFragmid fragment: (Cas protein) Cas7-11DepositorInsertCas7-11_v1.1 [Di]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA043
Plasmid#215964PurposeFragmid fragment: (Pol 2 promoter) human Phosphoglycerate Kinase promoterDepositorHas ServiceCloning Grade DNAInsertPGK_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA066
Plasmid#215966PurposeFragmid fragment: (Pol 2 promoter) human UbiquitinC promoterDepositorHas ServiceCloning Grade DNAInsertUbC_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA369
Plasmid#215987PurposeFragmid fragment: (N' terminus) attenuate expressionDepositorHas ServiceCloning Grade DNAInsertStart; u(S)ORF_v1.1; Stop; Start
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA181
Plasmid#216024PurposeFragmid fragment: (Cas protein) unmodified AsCas12a; not preferredDepositorInsertCas12a_v1.1 [As]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA197
Plasmid#216049PurposeFragmid fragment: (C' terminus) ALFA; ALFA; ALFA; ALFA; ALFA; NpNLS; 3xHADepositorInsertALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5; NpNLS_v1.1; 3xHA_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA365
Plasmid#215969PurposeFragmid fragment: (Pol 2 promoter) Gal4-induced expressionDepositorHas ServiceCloning Grade DNAInsertLoxP_v1; 5xUAS_v1; LoxP_v1; 5xUAS_v1; Hsp70_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA265
Plasmid#215967PurposeFragmid fragment: (Pol 2 promoter) spleen focus-forming virus promoterDepositorHas ServiceCloning Grade DNAInsertSFFV_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA371
Plasmid#215989PurposeFragmid fragment: (N' terminus) attenuate expressionDepositorHas ServiceCloning Grade DNAInsertStart; u(S)ORF_v1.1; Stop; Start; HIV-NES_v1.2; HIV-NES_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA416
Plasmid#215992PurposeFragmid fragment: (N' terminus) luminescent reporterDepositorHas ServiceCloning Grade DNAInsertStart; V5_v1.1; NanoLuc_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA195
Plasmid#215984PurposeFragmid fragment: (N' terminus) ALFA tag for recruiting via NbALFADepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA198
Plasmid#215985PurposeFragmid fragment: (N' terminus) activator from p65 subunit of mouse NfkbDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; p65_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA179
Plasmid#215939PurposeFragmid fragment: (guide cassette) short sequence with no known functionDepositorHas ServiceCloning Grade DNAInsertFiller
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA125
Plasmid#215982PurposeFragmid fragment: (N' terminus) activator from p65 subunit of mouse NfkbDepositorHas ServiceCloning Grade DNAInsertStart; p65_v1.3; HSF1_v1.2; Linker_v3.1; SV40NLS_v1.6
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA025
Plasmid#216005PurposeFragmid fragment: (Cas protein) NG PAMDepositorInsertCas9-NG_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA081
Plasmid#215977PurposeFragmid fragment: (N' terminus) HIV Nuclear Export SequenceDepositorHas ServiceCloning Grade DNAInsertStart; HIV-NES_v1.2; HIV-NES_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA027
Plasmid#216007PurposeFragmid fragment: (Cas protein) nearly PAM-lessDepositorInsertCas9-SpRY_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA124
Plasmid#215981PurposeFragmid fragment: (N' terminus) catalytic core of human acetyltransferase p300DepositorHas ServiceCloning Grade DNAInsertStart; p300_v1.2; Linker_v3.1; SV40NLS_v1.6
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA122
Plasmid#215980PurposeFragmid fragment: (N' terminus) 4xVP16 transcritional activatorsDepositorHas ServiceCloning Grade DNAInsertStart; VP64_v1.1; Linker_v3.1; SV40NLS_v1.6
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only