-
Plasmid#40851PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–mm14-21
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS-15 (mm15-21)
Plasmid#40850PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–mm15-21
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS14 (mm16-21)
Plasmid#40849PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–mm16-21
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS-12 (mm1-8)
Plasmid#40847PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–mm1-8
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS-11 (bulge 9-15)
Plasmid#40846PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–bulge9-15
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS-10 (bulge 9-13)
Plasmid#40845PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–bulge 9-13
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS-9 (bulge 9-11)
Plasmid#40844PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–bulge 9-11
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab9Db WT
Plasmid#37783DepositorInsertRab9Db (Rab9Db Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP RabX2 WT
Plasmid#37753DepositorInsertRabX2 (RabX2 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F15
Plasmid#23064DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 deleted amino acids 4 - 19PromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F23
Plasmid#23065DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysine 5 to GlutaminePromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F33
Plasmid#23068DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysine 5 to Glutamine H4 K5QPromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2 enhancer- F3 GATA mut
Plasmid#22430DepositorInsertF3 GATA mut (NOS3 Human)
UseLuciferaseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2 enhancer- F4 GATA mut
Plasmid#22435DepositorInsertF4 GATA mut (NOS3 Human)
UseLuciferaseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F47
Plasmid#23071DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysines 8 and 16 to Glutamines…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F49
Plasmid#23073DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysines 8 and 16 to Arginine, …PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F51
Plasmid#23075DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysines 5 and 12 to Glutamine,…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F52
Plasmid#23076DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysines 5 and 12 to Arginines,…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F36
Plasmid#23069DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 14 to Glycine, H3 K14GPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F43
Plasmid#23070DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 14 to Arginine, H3 K14RPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2 enhancer- F6 Sp-1 mut
Plasmid#22439DepositorInsertF6 Sp-1 mut (NOS3 Human)
UseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
XP31 Vox
Plasmid#16972DepositorInsertVox (ventx2.1.L Frog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-mVenus-p27K−
Plasmid#176651PurposeIt encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).DepositorInsertmVenus-p27K- (Cdkn1b Mouse)
UseLentiviralTagsmVenusExpressionMutationMutant K- (lacks affinity to Cdk)PromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_miniTurbo-C12orf49
Plasmid#155111Purposetransfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioIDDepositorInsertC12orf49 (SPRING1 Human)
UseTagsminiTurboExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_C12orf49-miniTurbo
Plasmid#155112Purposetransfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioIDDepositorInsertC12orf49 (SPRING1 Human)
UseTags3x Flag and miniTurboExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMG051 MBP-β-catenin-His, GST-CK1α
Plasmid#154072PurposeCo-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-HisDepositorUseTagsGST, His6, and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304_C12orf49-V5
Plasmid#155110Purposelentiviral plasmid for the exogneous expression of C-terminal V5-tagged C12orf49DepositorInsertC12orf49 (SPRING1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterCMV promoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCjeCas9
Plasmid#172215PurposeExpresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cellsDepositorInsertCas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
UseTagsHistagExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCjedCas9
Plasmid#172216PurposeExpresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cellsDepositorInsertcatalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
UseTagsHistagExpressionMutationchanged Asp 8 to Ala and His 559 to AlaPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mClover3_AAVS1_sgRNA_2
Plasmid#155099Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_AAVS1_sgRNA_2
Plasmid#155105Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS10-ribE-dddAI-FLAG
Plasmid#186562PurposeRegulated expression in Firmicutes of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)DepositorInsertdddAI
UseTagsFLAGExpressionBacterialMutationPromoterpSpac(hy)Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_AAVS1_sgRNA_2
Plasmid#155085Purposelentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_AAVS1_sgRNA_3
Plasmid#155089Purposelentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_PSMB2_sgRNA_1
Plasmid#155092Purposelentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene)DepositorInsertPSMB2_sgRNA_1 (PSMB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_PSMD1_sgRNA_1
Plasmid#155091Purposelentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene)DepositorInsertPSMD1_sgRNA_1 (PSMD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_PSMD1_sgRNA_1
Plasmid#155107Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)DepositorInsertPSMD1_sgRNA_1 (PSMD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_AAVS1_sgRNA_2
Plasmid#155088Purposelentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK1-R84S
Plasmid#213591PurposeDoxycycline inducible expression of myc-tagged ERK1-R84S cDNA in mammilian cellsDepositorInsertMAPK3 (MAPK3 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR84SPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK1-S170D
Plasmid#213592PurposeDoxycycline inducible expression of myc-tagged ERK1-S170D cDNA in mammilian cellsDepositorInsertMAPK3 (MAPK3 Human)
UseLentiviralTagsMYCExpressionMammalianMutationS170DPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mClover3_PSMD1_sgRNA_1
Plasmid#155102Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)DepositorInsertPSMD1_sgRNA_1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mClover3_AAVS1_sgRNA_4
Plasmid#155101Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_AAVS1_sgRNA_3
Plasmid#155086Purposelentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_AAVS1_sgRNA_4
Plasmid#155106Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_AAVS1_sgRNA_4
Plasmid#155087Purposelentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_AAVS1_sgRNA_4
Plasmid#155090Purposelentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)
Plasmid#134291PurposeExpresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acids 30, 280 and 443 to Asparag…PromoterT7Available sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-GK (281-473)
Plasmid#134273PurposeLentivector encoding Flag-tagged GK truncation (amino acids 281-473)DepositorInsertGk (Gk Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk amino acids 281-473PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-GK (12-272)
Plasmid#134272PurposeLentivector encoding Flag-tagged GK truncation (amino acids 12-272)DepositorInsertGk (Gk Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk amino acids 12-272PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5-toku (1-174)
Plasmid#134261PurposeLentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 1-174PromoterCMVAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only