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Plasmid#62684PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInserttdTomato/amiR-eGFP123/amiR-eGFP419
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATH (pKD-G6)
Plasmid#62685PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInserttdTomato/amiR-eGFP419/amiR-eGFP123
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATK (pKD-G9)
Plasmid#62688PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInserttdTomato/amiR-eGFP123/amiR-eGFP419
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevTre-mKLF5
Plasmid#41744DepositorInsertKlf5 (Klf5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(D77R/T214V)
Plasmid#66138PurposeExpresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationD77R, T214VPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 H4 (haplotype 4)
Plasmid#66718PurposeConstitutive expression of FPR1 in mammalian cellsDepositorInsertFPR1 (haplotype 4) (FPR1 Human)
UseTagsExpressionMammalianMutationPromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut2
Plasmid#51850Purposepromoter region of PIM2 lacking the forkhead binding site #1DepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorTagsExpressionMutationPromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
UAS-dar1-V5
Plasmid#45056DepositorInsertdar1 (dar1 Fly)
UseTagsV5ExpressionInsectMutationPromoterHS promoterAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-143
Plasmid#169923PurposeWT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2DepositorInsertEcm2 1-143
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-265
Plasmid#169925PurposeWT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2DepositorInsertEcm2 1-265
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-325
Plasmid#169926PurposeWT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2DepositorInsertEcm2 1-325
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pPEPY-PF6-lacI
Plasmid#85589PurposeWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniaeDepositorInsertlacI
UseTagsExpressionBacterialMutationPromoterPF6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHrD-IRES-Fluc
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
MP9956:SFG.GD3synthase-2A-GD2synthase
Plasmid#75013PurposeRetroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.DepositorInsertsmurine GD3 synthase
murine GD2 synthase
UseRetroviral; Momlv retroviral vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJWV102-PL-dCas9
Plasmid#85588PurposeIntegrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9spDepositorInsertdCas9sp
UseCRISPRTagsExpressionBacterialMutationPromoterPLspAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_GFP
Plasmid#96941PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertGreen fluorescent protein
UseLentiviralTagsExpressionMutationPromoterCaMKIIaAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p65-mNeonGreen
Plasmid#127172PurposeFluorescently tagged p65 reporterDepositorInsertGFP-RelA (RELA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOO2-GW
Plasmid#135097PurposeGateway vector for expression of proteins in Xenopus oocytesDepositorTypeEmpty backboneUseBacterial expression vector for generating sp6 tr…TagsExpressionMutationPromoterSP6Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Fuw-dCas9-Tet1CD
Plasmid#84475PurposeLentiviral construct to express dCas9 fused with the catalytic domain of Tet1DepositorInsertdCas9-Tet1CD
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_3E5E_luciferase
Plasmid#103055PurposeEncodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoterDepositorInsertEbolavirus minigenome
UseLuciferaseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-1: ORF5-TBP6.7-Pin domain-NLS
Plasmid#132543PurposeExpresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cellsDepositorInsertCIRTS-1: ORF5-TBP6.7-Pin domain-NLS
UseTagsFLAG tagExpressionMammalianMutationPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVDL9.3
Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Empty
Plasmid#91980PurposeEmpty backboneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(2A-9) pET29b
Plasmid#75145Purposereprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificityDepositorInsertreprogrammed sortase A variant
UseTags6x HisExpressionBacterialMutationS102C A104H E105D K138P K152I N160K K162H T164N K…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSP4-E228Q
Plasmid#80351PurposeExpression of GFP-tagged, dominant negative VPS4 mutantDepositorInsertVPS4-E228Q (VPS4A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-XKR8-n3xFLAG
Plasmid#215693PurposeProduces the XKR8 lipid scramblase with an n-terminal 3xFLAG tagDepositorInsertXKR8-n3xFLAG (XKR8 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR1D1_Hormone-recep
Plasmid#109960PurposeProtein expression and purification of NR1D1_Hormone-recepDepositorInsertNR1D1_Hormone-recep
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR6A1_Hormone-recep
Plasmid#109945PurposeProtein expression and purification of NR6A1_Hormone-recepDepositorInsertNR6A1_Hormone-recep
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hRARβP-FL-luciferase
Plasmid#135447PurposeHuman full-length promoter of retinoic acid receptor-beta gene in pGL3-Basic-luciferase plasmid vectorDepositorInsertFull-length promoter of human retinoic acid receptor-beta gene
UseTagsExpressionMutationPromoterHuman RARβ promoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR4A3_Hormone-recep
Plasmid#109952PurposeProtein expression and purification of NR4A3_Hormone-recepDepositorInsertNR4A3_Hormone-recep
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RORB_Hormone-recep
Plasmid#109958PurposeProtein expression and purification of RORB_Hormone-recepDepositorInsertRORB_Hormone-recep
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p_m16S
Plasmid#160549PurposeUsed for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: CarbenicillineDepositorInsert16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-2xAM
Plasmid#92220PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sitesDepositorInsertsSp-dCas9-2xAM tag
gRNA to be inserted into Bbs I sites
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6Available sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP35_EBOV
Plasmid#103050PurposeEncodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_L_EBOV
Plasmid#103052PurposeEncodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertL gene of Ebola virus (L Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP30_EBOV
Plasmid#103051PurposeEncodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_VHH-GFP4::CD8::mCherry
Plasmid#163917PurposeExpression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancersDepositorInsertFusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SUFU_Sufu
Plasmid#178488PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertSUFU_Sufu (SUFU Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only