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Plasmid#160293Purposetarget Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.DepositorInsertPer2LUC
UseTagsExpressionMammalianMutationPromoterno promotorAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD108
Plasmid#119884PurposeIntegration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance markerDepositorInsertBbaJ23104_EYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB061i3
Plasmid#79230PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAF0033 Ef1a-Pa01-2a-EGFP
Plasmid#193460PurposeExpression of RecombinaseDepositorInsertPa01
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF-HdrB3
Plasmid#184908Purposecarrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferriduransDepositorInsertCas9-sgRNA-homology arms
UseCRISPR; Broad-host-rangeTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Flag-NSF
Plasmid#74924PurposeMammalian expression of human NSF full-length (744 aa)DepositorInsertNSF (NSF Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CARPID BASU-dCasRx
Plasmid#153209Purposeexpress CARPID BASU-dCasRx fusion protein in mammalian cellsDepositorInsertBASU-dCasRx
UseCRISPR and LentiviralTagsExpressionMammalianMutationR239A/H244A/R858A/H863A in RfxCas13dPromoterEF-1aAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC11A2_STOP
Plasmid#161050PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC11A2 (SLC11A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOCC5
Plasmid#118863Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP71-GM-CSF
Plasmid#174596Purposeexpresses secreted murine GM-CSFDepositorInsertGM-CSF
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-PdCO-mScarlet
Plasmid#198507PurposeExpresses optimized PdCO in frame with mScarlet under control of CMV promotor.DepositorInsertPdCO
UseTagsRho1D4 and mScarletExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
R979-X58-008: Hs.KRAS4b(1-188)
Plasmid#159691PurposeBaculovirus protein expression of KRAS4b (1-188)DepositorInsertHs.KRAS4b(1-188) (KRAS Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_blast-tagBFP-lox2272
Plasmid#162071Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN-EphA2-Flag
Plasmid#102755PurposeExpression of EphA2DepositorInsertEphA2 (EPHA2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
proE-cad178-Luc
Plasmid#42081DepositorInsertE-cadherin (CDH1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains E-cadherin promoter region from -178 to …PromoterE-cadherinAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF2651
Plasmid#144058PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTERB1 (TERB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCS4 3XFLAG-PPARgamma2
Plasmid#78770PurposeTo overexpress PPARgamma2 in Mammalian CellsDepositorInsertPPAR_2 (Ppargc1a Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94Available sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-Yap shRNA
Plasmid#166486PurposeMouse Yap RNAiDepositorInsertshRNA for mouse Yap (Yap1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TurboID-Cyclin D1
Plasmid#215084PurposeExpresses TurboID-Cyclin D1 in mammalian cells from a retroviral vector.DepositorInsertCCND1 (CCND1 Human)
UseRetroviralTagsTurboIDExpressionMammalianMutationPromoterLTRAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only