Addgene

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pEBG‐AMPK α1(1‐312)

Plasmid

#27632

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

AMPK α1(1‐312) (Prkaa1 Rat)

Use

Tags

GST

Expression

Mammalian

Mutation

Constitutively  active  by  truncation  after  am…

Promoter

Available since

May 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

Plasmid

#171436

Purpose

Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-Lifeact

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET30-HPF1-His-Sumo-Flag

Plasmid

#111577

Purpose

Expresses full length HPF1 in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

HPF1 (HPF1 Human)

Use

Tags

6xHis-Sumo-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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lvl0 pOp6-35S

Plasmid

#153409

Purpose

Dex system inducible promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

pOp6-35S promoter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-XBP1s

Plasmid

#63680

Purpose

Mammalian expression of Flag tagged XBP1s

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL701

Plasmid

#140371

Purpose

Expresses TetR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-TetR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Expression

Mutation

Promoter

UbC

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHyo245

Plasmid

#173147

Purpose

Expresses pheS_A294G by dual T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pheS_A294G

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-HBx

Plasmid

#24931

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT

Plasmid

#26106

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Type

Empty backbone

Use

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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