Addgene

We narrowed to 2,808 results for: sam

Showing: 1401 - 1420 of 2808 results

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pMXS-hs-3xHA-LIN-41

Plasmid

#52717

Purpose

retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP_IRES_tdTomato

Plasmid

#184046

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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3XFlag-pA-Tn5-Fl

Plasmid

#124601

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Steven Henikoff

Article

Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

Insert

Protein A and hyperactive Tn5 transposase (Tnp) fusion protein

Use

Tags

Protein A and Tn5 transposase fusion protein with…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-metap2

Plasmid

#100512

Purpose

Expresses HA tagged metap2 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pNS35 (multiAsCas12a piggyBac)

Plasmid

#217336

Purpose

piggyBac expression of multiAsCas12a

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-P2A-TagBFP2

Expression

Mammalian

Mutation

R1226A/E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMIEG3-c-Jun

Plasmid

#40348

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

c-Jun (JUN Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-LDLR

Plasmid

#162717

Purpose

Mammalian expression of human LDLR

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

LDLR (LDLR Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMIEG3-JunDN

Plasmid

#40350

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

Jun dominant negative (JUN Human)

Use

Retroviral

Tags

HA and IRES eGFP

Expression

Mammalian

Mutation

first 122 amino acids deleted

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pMX-hTAF7

Plasmid

#44338

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF7 (TAF7 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pMX-hTAF10

Plasmid

#44340

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF10 (TAF10 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only

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pCH61 (multiAsCas12a-KRAB piggyBac)

Plasmid

#217332

Purpose

piggyBac expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

R1226A/E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-wildtype

Plasmid

#31972

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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PLX_307 GFP

Plasmid

#184492

Purpose

Lentivirus expression for GFP

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2A_tdTomato

Plasmid

#184045

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJH3628

Plasmid

#191445

Purpose

Prgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pregf-1

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Tags

Expression

Mutation

Promoter

DHFR-TS 5' UTR

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro

Plasmid

#31845

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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PLX306 iCre tetO tomato rtTA

Plasmid

#158050

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

tdTomato

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

tetO promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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